Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Recombinant human HGF protein (rp175908) with the ED50 is 97.73 ng/mL.
Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: HGF R, also known as Met (from N-methyl-N’-nitro-N-nitrosoguanidine induced), is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGF R is synthesized as a single chain precursor which undergoes cotranslational proteolytic cleavage. This generates a mature HGF R that is a disulfide-linked dimer composed of a 50 kDa extracellular alpha chain and a 145 kDa transmembrane beta chain. The extracellular domain (ECD) contains a seven bladed beta-propeller sema domain, a cysteine-rich PSI/MRS, and four Ig-like E-set domains, while the cytoplasmic region includes the tyrosine kinase domain. Proteolysis and alternate splicing generate additional forms of human HGF R which either lack of the kinase domain, consist of secreted extracellular domains, or are deficient in proteolytic separation of the alpha and beta chains. The sema domain, which is formed by both the alpha and beta chains of HGF R, mediates both ligand binding and receptor dimerization. Ligand-induced tyrosine phosphorylation in the cytoplasmic region activates the kinase domain and provides docking sites for multiple SH2-containing molecules. HGF stimulation induces HGF R down-regulation via internalization and proteasome-dependent degradation. In the absence of ligand, HGF R forms noncovalent complexes with a variety of membrane proteins including CD44v6, CD151, EGF R, Fas, Integrin alpha 6/beta 4, Plexins B1, 2, 3, and MSP R/Ron. Ligation of one complex component triggers activation of the other, followed by cooperative signaling effects. Formation of some of these heteromeric complexes is a requirement for epithelial cell morphogenesis and tumor cell invasion. Paracrine induction of epithelial cell scattering and branching tubulogenesis results from the stimulation of HGF R on undifferentiated epithelium by HGF released from neighboring mesenchymal cells. Genetic polymorphisms, chromosomal translocation, over-expression, and additional splicing and proteolytic cleavage of HGF R have been described in a wide range of cancers. Within the ECD, human HGF R shares 86%-88% aa sequence identity with canine, mouse, and rat HGF R.
规格
产品名称
Recombinant Human HGFR/c-MET Protein
别名
重组人HGFR/c-MET蛋白 | 重组人 HGFR/c-MET 蛋白
英文别名
AUTS9 | cMET | c-MET | hepatocyte growth factor receptor | HGF R | HGF receptor | HGF/SF receptor | HGFR | Met (c-Met) | met proto-oncogene (hepatocyte growth factor receptor) | met proto-oncogene tyrosine kinase | MET | oncogene MET | Proto-oncogene c-Met | RCCP2 | Scatter factor receptor | SF receptor | Tyrosine-protein kinase Met
Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Recombinant human HGF protein (rp175908) with the ED50 is 97.73 ng/mL.
36.0-50.0 & 72.0-92.0, under reducing condition; 110.2-155.6 kDa, under non-reducing condition
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 4% Trehalose, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human HGFR/c-MET Protein (rp169642) - Protein Bioactivity Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Recombinant human HGF protein (rp175908) with the ED₅₀ is 97.73 ng/mL.
Recombinant Human HGFR/c-MET Protein (rp169642) - SDS-PAGE 3 μg/lane of Recombinant Human HGFR/c-MET Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 36.0-50.0 & 72.0-92.0 kDa under reducing conditions and 110.2-155.6 kDa under non-reducing conditions. Heterodimer made of an alpha chain and a beta chain which are disulfide linked (Uniprot: P08581).
ZJ24F0505921
危险品化学品经营许可证（带存储）