Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.
Post-translational: Directly cleaved by HTRA2 to produce active form.
生物活性
Immobilized Recombinant Human MMP-3 Protein (rp169644) at 1.0 μg/mL can bind MMP3 Mouse mAb (Ab176193) with the EC50 of 76.2 ng/mL.
53.4-59.7 kDa, under reducing conditions; 50.4-56.2 & 110.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM Tris, 150mM NaCl, 5% Glycerol, pH 7.5.
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human MMP-3 Protein (rp169644) - ELISA Immobilized Recombinant Human MMP-3 Protein (rp169644) at 1.0 μg/mL can bind MMP3 Mouse mAb (Ab176193) with the EC50 of 76.2 ng/mL.
Recombinant Human MMP-3 Protein (rp169644) - SDS-PAGE 3 μg/lane of Recombinant Human MMP-3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 53.4-59.7 kDa under reducing conditions and 50.4-56.2 & 110.8 kDa under non-reducing conditions.