CCL21, also known as 6Ckine, TCA-4, SLC, Exodus-2, and A21, is a 12 kDa homeostatic chemokine that plays an important role in adaptive immune responses and inflammation. Unlike other CC chemokines, human CCL21 has a 37 amino acid (aa) C-terminal extension which mediates its attachment to carbohydrate structures and extracellular matrix components. Mature human CCL21 shares 71% and 66% aa sequence identity with mouse and rat CCL21, respectively. Both human and mouse CCL21 signal through the chemokine receptor CCR7, while mouse CCL21 additionally can signal through CXCR3. CCL21 is constitutively presented on initial lymphatic vessels, high endothelial venules (HEV), and lymph node dendritic cells (DC). Immobilized CCL21 promotes the docking of DC to lymphatic vessels and the retention of T cells by lymph node DC, resulting in T cell priming for activation. DC interaction with the anchored chemokine can induce CCL21 cleavage and release of an 8 kDa fragment that lacks the C-terminal extension. During chronic inflammation or tissue damage, CCL21 is up‑regulated on local vascular endothelial cells, macrophages, T cells, and neurons. In these settings, it promotes fibrosis, inflammatory cytokine production, and neuropathic pain. The soluble chemokine is elevated in rheumatoid arthritis synovial fluid and in the serum of coronary artery disease patients. CCL21 has been shown to exert either angiogenic or angiostatic effects. These effects, in combination with the ability of CCL21 to attract immune suppressor cells (Treg and MDSC) to a tumor site, can have positive or negative effects on tumor progression.
生物活性
Determined by its ability to chemoattract human total lymphocyte population using a concentration range of 10.0-100.0 ng/mL.
15.5 kDa, under reducing conditions; 15.5 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human CCL21/6Ckine Protein - Bioactivity Determined by its ability to chemoattract human total lymphocyte population using a concentration range of 10.0-100.0 ng/mL.
Recombinant Human CCL21/6Ckine Protein - SDS-PAGE 3 μg/lane of Recombinant Human CCL21/6Ckine Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.5 kDa.