Immobilized Recombinant Human CXCL4/PF4 Protein (rp170411) at 2.0 μg/mL can bind U.Penn. patent anti-PF4 (anti-CXCL4) (Ab209769) with the EC50 of 74.0 ng/mL.
Platelet factor 4 (PF4), also known as chemokine (C-X-C motif) ligand 4 (CXCL4), is a small cytokine belonging to the CXC chemokine family. CXCL4/PF4 is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, CXCL4/PF4 probably has a role in inflammation and wound repair. This protein is released during platelet aggregation. CXCL4/PF4 neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 is chemotactic for neutrophils and monocytes. It inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. CXCL4/PF4 is up-regulated in human liver fibrosis and that it plays a nonredundant, functional role in experimental liver fibrosis by mediating stellate cell proliferation, migration, and intrahepatic immune cell recruitment.
生物活性
Immobilized Recombinant Human CXCL4/PF4 Protein (rp170411) at 2.0 μg/mL can bind U.Penn. patent anti-PF4 (anti-CXCL4) (Ab209769) with the EC50 of 74.0 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human CXCL4/PF4 Protein (rp170411) - ELISA Immobilized Recombinant Human CXCL4/PF4 Protein (rp170411) at 2.0 μg/mL can bind U.Penn. patent anti-PF4 (anti-CXCL4) (Ab209769) with the EC50 of 74.0 ng/mL.
Recombinant Human CXCL4/PF4 Protein (rp170411) - SDS-PAGE 3 μg/lane of Recombinant Human CXCL4/PF4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 11.1 kDa . The observation of CXCL4/PF4 Protein as both dimers and monomers under under reducing (R) and non-reducing (N) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds (Uniprot: P02776.2).