exodus-1 | LARC | MIP-3 alpha | MIP3 alpha | S Small inducible cytokine A20 precursor | CCL20 | Macrophage inflammatory protein 3 alpha | Liver and activation- regulated chemokine | CC chemokine LARC | Beta chemokine exodus-1 | CKb4 | ST38 | MIP3A | MIP-3
规格或纯度
无载体,生物活性,ActiBioPure™,高性能,His标签,≥95%(SDS-PAGE),expressed in E. coli; 见COA
生化机理
Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:11035086, PubMed:11352563, PubMed:20068036).
The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases (PubMed:21376174).
CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (PubMed:11352563, PubMed:9038201).
Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for optimal migration of thymic natural regulatory T cells (nTregs) and DN1 early thymocyte progenitor cells (By similarity).
C-terminal processed forms have been shown to be equally chemotactically active for leukocytes (PubMed:11035086).
Positively regulates sperm motility and chemotaxis via its binding to CCR6 which triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed:23765988, PubMed:25122636).
Inhibits proliferation of myeloid progenitors in colony formation assays (PubMed:9129037).
May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells (By similarity).
Possesses antibacterial activity towards E.coli ATCC 25922 and S.aureus ATCC 29213 (PubMed:12149255).
Post-translational: C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
生物活性
Determined by its ability to chemoattract human T cells using a concentration of 10.0-50.0 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human CCL20/MIP-3 alpha Protein (rp170424) - Protein Bioactivity
Determined by its ability to chemoattract human T cells using a concentration of 10.0-50.0 ng/mL.
Recombinant Human CCL20/MIP-3 alpha Protein (rp170424) - SDS-PAGE
2 μg/lane of Recombinant Human CCL20/MIP-3 alpha Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 26.7 & 50.6 kDa under reducing conditions and 27.0 & 51.1 & 98.3 & 144.0 & 202.7 & 250.0 kDa under non-reducing conditions.
CCL20/MIP-3 alpha may form polymers mediated by disulfide bonds (Uniprot: P78556).
ZJ26F0434760
危险品化学品经营许可证（带存储）