1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC50 for this effect is 97.73 ng/mL.
HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It's a multidomain molecule with an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain lacking detectable protease activity. Human HGF is secreted as an inactive single chain propeptide and cleaved to form a bioactive disulfide-linked molecule with alpha and beta chains. Alternate splicing generates human HGF isoforms with variations in the Kringle domains. Human HGF shares high amino acid sequence identity with other mammals. HGF binds to heparan-sulfate proteoglycans and the receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in cancer development. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It induces the up-regulation of integrin alpha 2 beta 1 in epithelial cells and can alter epithelium morphology. In the thyroid, HGF influences thyrocyte behavior and inhibits iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.
Post-translational: The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
生物活性
1. Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED50 for this effect is 24.77 ng/mL. 2. Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC50 for this effect is 97.73 ng/mL.
30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa, under reducing condition; 67.0-77.2 kDa, under non-reducing condition
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human HGF Protein (rp175908) - Protein Bioactivity Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The ED
₅₀
for this effect is 24.77 ng/mL.
Recombinant Human HGF Protein (rp175908) - Protein Bioactivity Immobilized Recombinant Human HGFR/c-MET Protein (rp169642) at 1.0 μg/mL can bind Biotinylated Recombinant Human HGF Protein (rp175908) with the EC
₅₀
for this effect is 97.73 ng/mL.
Recombinant Human HGF Protein (rp175908) - ELISA Immobilized Recombinant Human HGF Protein (rp156180) at 1.0 μg/mL can bind Ficlatuzumab (anti-HGF) (Ab170563) with the EC
₅₀
of 45.64 ng/mL.
Recombinant Human HGF Protein (rp175908) - ELISA Immobilized Recombinant Human HGF Protein (rp156180) at 1.0 μg/mL can bind Rilotumumab (anti-HGF) (Ab170950) with the EC
₅₀
of 21.92 ng/mL.
Recombinant Human HGF Protein (rp175908) - SDS-PAGE 2 μg/lane of Recombinant Human HGF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.0-40.0 kDa & 56.0-65.0 kDa & 85.0-95.0 kDa under reducing conditions and 67.0-77.2 kDa under non-reducing conditions.
ZJ24F0606556
危险品化学品经营许可证（带存储）