1. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Toripalimab (anti-PD-1) (Ab182801) with the EC50 of 13.47 ng/mL. 2. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Zimberelimab (anti-PD-1) (Ab175710) with the EC50 of 19.95 ng/mL. 3. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Retifanlimab (anti-PD-1) (Ab175644) with the EC50 of 11.39 ng/mL. 4. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Dostarlimab (anti-PD-1) (Ab175513) with the EC50 of 32.46 ng/mL. 5. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Spartalizumab (anti-PD-1) (Ab169299) with the EC50 of 12.83 ng/mL. 6. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Camrelizumab (anti-PD-1) (C412007) with the EC50 of 10.39 ng/mL. 7. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Sintilimab (anti-PD-1) (Ab169307) with the EC50 of 15.02 ng/mL. 8. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Cemiplimab (anti-PD-1) (C412010) with the EC50 of 12.82 ng/mL.
Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Programmed Death-1 receptor (PD-1), also known as CD279, is a type I transmembrane protein belonging to the CD28 family of immune regulatory receptors. Other members of this family include CD28, CTLA-4, ICOS, and BTLA. Mature human PD-1 consists of a 148 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 24 aa transmembrane domain, and a 95 aa cytoplasmic region. The human PD-1 ECD shares 65% aa sequence identity with the mouse PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes. Ligation of PD-L1 with PD-1 induces co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion. Thus, the PD-1: PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance. Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy.
规格
产品名称
Recombinant Human PD-1 Protein
别名
重组人PD-1蛋白 | 重组人PD-1蛋白
英文别名
PDCD1_HUMAN | Programmed cell death 1 | Programmed cell death 1 protein | Programmed cell death protein 1 | Protein PD 1 | Protein PD-1 | SLEB2 | Systemic lupus erythematosus susceptibility 2 | CD279 | CD279 antigen | hPD 1 | hPD l | hPD-1 | hSLE1 | PD 1
规格或纯度
无动物源,无载体,生物活性,His标签,PBS Only,≥95%(SDS-PAGE)
生物活性
1. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Toripalimab (anti-PD-1) (Ab182801) with the EC50 of 13.47 ng/mL. 2. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Zimberelimab (anti-PD-1) (Ab175710) with the EC50 of 19.95 ng/mL. 3. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Retifanlimab (anti-PD-1) (Ab175644) with the EC50 of 11.39 ng/mL. 4. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Dostarlimab (anti-PD-1) (Ab175513) with the EC50 of 32.46 ng/mL. 5. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Spartalizumab (anti-PD-1) (Ab169299) with the EC50 of 12.83 ng/mL. 6. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Camrelizumab (anti-PD-1) (C412007) with the EC50 of 10.39 ng/mL. 7. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Sintilimab (anti-PD-1) (Ab169307) with the EC50 of 15.02 ng/mL. 8. Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Cemiplimab (anti-PD-1) (C412010) with the EC50 of 12.82 ng/mL.
38.6-44.2 kDa, under reducing conditions; 37.0-43.5 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Toripalimab (anti-PD-1) (Ab182801) with the EC₅₀ of 13.47 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Zimberelimab (anti-PD-1) (Ab175710) with the EC₅₀ of 19.95 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Retifanlimab (anti-PD-1) (Ab175644) with the EC₅₀ of 11.39 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Dostarlimab (anti-PD-1) (Ab175513) with the EC₅₀ of 32.46 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Spartalizumab (anti-PD-1) (Ab169299) with the EC₅₀ of 12.83 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Camrelizumab (anti-PD-1) (C412007) with the EC₅₀ of 10.39 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Sintilimab (anti-PD-1) (Ab169307) with the EC₅₀ of 15.02 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 Protein (rp176241) at 1.0 μg/mL can bind Cemiplimab (anti-PD-1) (C412010) with the EC₅₀ of 12.82 ng/mL.
Recombinant Human PD-1 Protein (rp176241) - SDS-PAGE 2 μg/lane of Recombinant Human PD-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.6-44.2 kDa under reducing conditions and 37.0-43.5 kDa under non-reducing conditions.
Recombinant Human PD-1 Protein (rp176241) - ELISA Immobilized Recombinant Human PD-1 protein (rp176241) at 1.0 μg/mL can bind Balstilimab (anti-PD-1) (Ab182812) with the EC
₅₀
of 16.49 ng/mL.
ZJ24F0606482
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