ActiBioPure™,His标签,PBS Only,无动物源,无叠氮钠,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining
Description:
Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V‑type and one C‑type Ig domain, a transmembrane segment and a short cytoplasmic tail. Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern. It would appear that the human 4Ig form is the principal, if not the only form of B7-H3. Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V‑type and C‑type, and apparently are the consequence of a V‑C type tandem duplication. B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells. The mouse gene differs from that of human in that it cannot code for four Ig-like domains; only a V‑type:C‑type pair. Human B7-H3b binding to an undefined receptor has shown to be inhibitory to NK cell illing and cytokine release. It also seems to be required for late stage osteoblast differentiation.
ELISA: Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244)
Measured by its binding ability in a functional ELISA. Immobilized CD276 mouse mAb at 1.0 μg/mL can bind Recombinant Human B7-H3 (4Ig)/B7-H3b Protein (rp176244) with the EC₅₀ of 51.95 ng/mL.
Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized human Human B7-H3 (4Ig)/B7-H3b (rp176244) at 1.0 μg/mL can bind CD276 mouse mAb with the EC₅₀ of 1.86 ng/mL.
Recombinant Human B7-H3(4Ig)/B7-H3b Protein (rp176244) - SDS-PAGE
3 μg/lane of Recombinant Human B7-H3(4Ig)/B7-H3b Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 63.2 - 82.3 kDa under reducing conditions and 58.6 - 73.9 kDa under non-reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0202912 |
分析证书 | rp176244 |
| ZJ24F0202913 |
分析证书 | rp176244 |
| ZJ24F0202914 |
分析证书 | rp176244 |
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