Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 27.85 ng/mL. Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC50 of 41.55 ng/mL.
Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Tumor necrosis factor receptor superfamily, member 10b, official symbol TNFRSF10B, also known as Death receptor 5, CD262, TNF-related apoptosis-inducing ligand receptor 2 (TRAIL R2), is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R2/CD262/TNFRSF10B was purified independently as the only receptor for TRAIL detectable on the surface of two different human cell lines that undergo apoptosis upon stimulation with TRAIL. TRAIL R2/CD262/TNFRSF10B contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain. TRAIL R2/CD262/TNFRSF10B mediates apoptosis via the intracellular adaptor molecule FADD/MORT1. TRAIL receptors can signal both death and gene transcription, functions reminiscent of those of TNFR1 and TRAMP, two other members of the death receptor family. Defects in TRAIL R2/CD262/TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) also known as squamous cell carcinoma of the head and neck.
规格
产品名称
Recombinant Human TRAIL R2/TNFRSF10B Protein
别名
重组人TRAIL R2/TNFRSF10B蛋白 | 重组人TRAIL R2/TNFRSF10B蛋白
英文别名
CD262 antigen | CD262 | death domain containing receptor for TRAIL/Apo-2L | Death receptor 5 | DR5 | DR5TRICK2B | Fas-like protein | KILLER/DR5 | KILLERapoptosis inducing protein TRICK2A/2B | TNF-related apoptosis-inducing ligand receptor 2 | TNFRSF10B |
规格或纯度
无动物源,无载体,生物活性,高性能,His标签,PBS Only,≥95%(SDS-PAGE)
生物活性
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED50 for this effect is 27.85 ng/mL. Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC50 of 41.55 ng/mL.
14.7&18.7 kDa, under reducing conditions; 13.7&17.5 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693)- Protein Bioactivity
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) inhibits Recombinant Human TRAIL mediated cytotoxicity using L-929 mouse fibroblast cells. The ED₅₀ for this effect is 27.85 ng/mL.
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) - ELISA Immobilized Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) at 5.0 μg/mL can bind Recombinant Human TRAIL Protein (rp169692) with the EC₅₀ of 41.55 ng/mL.
Recombinant Human TRAIL R2/TNFRSF10B Protein (rp176693) - SDS-PAGE 3 μg/lane of Recombinant Human TRAIL R2/TNFRSF10B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.7&18.7 kDa under reducing conditions and 13.7&17.5 kDa under non-reducing conditions.
ZJ24F0606719
危险品化学品经营许可证（带存储）