Recombinant Human TIM-3 Protein

Name: Recombinant Human TIM-3 Protein
SKU: rp176703
Availability: InStock

货号: rp176703

COA

有货

级别和纯度: 无动物源 ? 无载体 ? 生物活性 ? ActiBioPure™ ? His标签 ? PBS Only ? ≥90%(SDS-PAGE)

Accession #

Q8TDQ0

蛋白标签

C-His

表达系统

HEK293

内毒素水平

<1.0 EU/μg

生物活性

Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.

规格

库存

价格

数量

10μg

rp176703-10μg

期货

¥539.90

50μg

rp176703-50μg

期货

¥1,599.90

100μg

rp176703-100μg

期货

¥2,619.90

1mg

rp176703-1mg

期货

¥16,999.90

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概述规格储存与运输图片高级数据质检证书(CoA,COO,BSE/TSE 和分析图谱) 溶液计算器常见问题

概述

Protein Function:：
Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.

规格

产品名称

Recombinant Human TIM-3 Protein

别名

重组人TIM-3蛋白 | 重组人类 TIM-3 蛋白

英文别名

规格或纯度

无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥90%(SDS-PAGE)

生物活性

表达系统

HEK293

种属

人(Human)

氨基酸

1-200 aa

序列

MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRHHHHHH

蛋白标签

C-His

无载体

Yes

无动物源

Yes

来源

重组表达

预测分子量

20.7 kDa

SDS-PAGE

40.0-50.0 kDa, under reducing conditions; 48.0 & 65.0-90.0 kDa, under non-reducing conditions

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

在 -20°C 下可保存 1 年。避免冷冻/解冻循环。

图片

Binding Activity: Recombinant Human TIM-3 Protein (rp176703)

Recombinant Human TIM-3 Protein (rp176703) - Binding Activity
Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC₅₀ of 1.267 μg/mL.

ELISA: Recombinant Human TIM-3 Protein (rp176703)

Recombinant Human TIM-3 Protein (rp176703) - ELISA
Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC₅₀ of 15.3 ng/mL.

SDS-PAGE: Recombinant Human TIM-3 Protein (rp176703)

Recombinant Human TIM-3 Protein (rp176703) - SDS-PAGE
3 μg/lane of Recombinant Human TIM-3 Protein was resolved with SDS-PAGE under reducing (R) and no-reducing (N) and visualized by Coomassie® Blue staining, showing a band at 40.0-50.0 kD under reducing conditions and 48.0 kDa and 65.0-90.0 kDa under non-reducing conditions.

高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

常见问题

本产品的纯度是多少？如何测定？

本产品的纯度为 ≥90%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。

什么是「无动物源」？

「无动物源」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

什么是「无载体」？

「无载体」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

本产品应如何储存？

请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。

本产品如何运输？

本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。

如何获取批次专用COA？

每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 His Tag 级别指南 → 查看 PBS Only 级别指南 →

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。

Recombinant Human TIM-3 Protein

概述

规格

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

常见问题

需要帮助选择级别？

查看相关“主题”系列

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