Recombinant Human TIM-3 Protein

无动物源

无载体

生物活性

ActiBioPure™

≥90%(SDS-PAGE)

功能和特点

表达系统: HEK293
Accession #: Q8TDQ0
蛋白标签: C-His
生物活性: Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp176703-10μg	10μg	期货
rp176703-50μg	50μg	期货
rp176703-100μg	100μg	期货
rp176703-1mg	1mg	期货

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点击查看蛋白信息: HAVCR2

Accession#:Q8TDQ0 (10) Gene ID:84868 (10) HAVCR2 (11) TIM-3 (7)

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基本描述

产品名称	Recombinant Human TIM-3 Protein
别名	重组人TIM-3蛋白 \| 重组人类 TIM-3 蛋白
英文别名	FLJ14428 \| HAVCR2 \| HAVcr-2 \| hepatitis A virus cellular receptor 2 \| kidney injury molecule-3 \| KIM-3 \| SPTCL \| T cell immunoglobulin mucin 3 \| CD366 \| T cell immunoglobulin mucin-3 \| T-cell immunoglobulin and mucin domain-containing protein 3 \| TIM 3 \|
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, ≥90%(SDS-PAGE)
产品介绍	Protein Function:： Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
生物活性	Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-200 aa
序列	MFSHLPFDCVLLLLLLLLTRSSEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q8TDQ0
来源	重组表达
预测分子量	20.7 kDa
SDS-PAGE	40.0-50.0 kDa, under reducing conditions; 48.0 & 65.0-90.0 kDa, under non-reducing conditions

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Binding Activity: Recombinant Human TIM-3 Protein (rp176703)

Recombinant Human TIM-3 Protein (rp176703) - Binding Activity
Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC₅₀ of 1.267 μg/mL.

ELISA: Recombinant Human TIM-3 Protein (rp176703)

Recombinant Human TIM-3 Protein (rp176703) - ELISA
Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC₅₀ of 15.3 ng/mL.

SDS-PAGE: Recombinant Human TIM-3 Protein (rp176703)

Recombinant Human TIM-3 Protein (rp176703) - SDS-PAGE
3 μg/lane of Recombinant Human TIM-3 Protein was resolved with SDS-PAGE under reducing (R) and no-reducing (N) and visualized by Coomassie® Blue staining, showing a band at 40.0-50.0 kD under reducing conditions and 48.0 kDa and 65.0-90.0 kDa under non-reducing conditions.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

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Recombinant Human TIM-3 Protein

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