Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.
Protein Function:： Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both the mouse and human. Recently, the identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In the tumor rejection model, the soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in the tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.
规格
产品名称
Recombinant Human TIM-3 Protein
别名
重组人TIM-3蛋白 | 重组人类 TIM-3 蛋白
英文别名
FLJ14428 | HAVCR2 | HAVcr-2 | hepatitis A virus cellular receptor 2 | kidney injury molecule-3 | KIM-3 | SPTCL | T cell immunoglobulin mucin 3 | CD366 | T cell immunoglobulin mucin-3 | T-cell immunoglobulin and mucin domain-containing protein 3 | TIM 3 |
Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC50 of 1.267 μg/mL. Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC50 of 15.3 ng/mL.
40.0-50.0 kDa, under reducing conditions; 48.0 & 65.0-90.0 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human TIM-3 Protein (rp176703) - Binding Activity Immobilized Recombinant Human Galectin-9 Protein at 1.0 μg/mL can bind Recombinant Human TIM-3 Protein (rp176703) with the EC₅₀ of 1.267 μg/mL.
Recombinant Human TIM-3 Protein (rp176703) - ELISA Immobilized Recombinant Human TIM-3 Protein (rp176703) at 1.0 μg/mL can bind Surzebiclimab (anti-TIM-3) (Ab182869) with the EC₅₀ of 15.3 ng/mL.
Recombinant Human TIM-3 Protein (rp176703) - SDS-PAGE 3 μg/lane of Recombinant Human TIM-3 Protein was resolved with SDS-PAGE under reducing (R) and no-reducing (N) and visualized by Coomassie® Blue staining, showing a band at 40.0-50.0 kD under reducing conditions and 48.0 kDa and 65.0-90.0 kDa under non-reducing conditions.
危险品化学品经营许可证（带存储）