Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description: Cyclophilin B (SCYLP, CyPB, and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, it mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization and facilitating the activity of additional chaperones. The human CyPB precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204), and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical.
22.6 kDa, reducing conditions; 22.6 and 45.6 kDa, non-reducing condition
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 100mM Imidazole, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human Cyclophilin B Protein (rp176704) - SDS-PAGE 3 μg/lane of Recombinant Human Cyclophilin B was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 22.6 kDa under reducing conditions and 22.6 & 45.6 kDa under non-reducing conditions.
Recombinant Human Cyclophilin B Protein (rp176704) - ELISA Immobilized Recombinant Human Cyclophilin B Protein (rp176704) at 1.0 μg/mL can bind Cyclophilin B Mouse mAb (Ab098512) with the EC₅₀ of 84.8 ng/mL.