Purity:>98%, by SDS-PAGE visualized with Coomassie® Blue Staining.

Description：

Heme oxygenase (HMOX) is the rate limiting enzyme in heme catabolism. It cleaves heme to biliverdin, carbon monoxide, and iron. The biliverdin is subsequently converted to bilirubin by biliverdin reductase. The mechanism of HMOX is unique in that heme serves as the substrate of the enzyme and as the prosthetic group for the activation of iron-bound O2. HMOX activity is highest in spleen where senescent erythrocytes are sequestered and destroyed. Two isoforms, HMOX1 and HMOX2, are expressed in most tissues. HMOX1 is an inducible enzyme in response to heme, heavy metals, oxidative stress, cytokines, and many drugs. Whereas HMOX2 displays a constitutive expression. HMOX1 is expressed mainly in spleen, liver, and kidney, and HMOX2 is prominently expressed in the brain and testes. The increased expression of HMOX1 levels is related to a variety of pathological states, where it functions as a cytoprotective molecule through its by products. HMOX1 also plays important roles in the regulation of cell proliferation, differentiation, and apoptosis.

规格

产品名称

Recombinant Human Heme Oxygenase 1 Protein

别名

重组人血红素加氧酶 1 蛋白

英文别名

heat shock protein, 32-kD | heme oxygenase (decycling) 1 | HMOX1 | HO | HO1 | HO-1 | HO-1heme oxygenase 1 | HSP32

规格或纯度

无载体,无叠氮钠,His标签,≥98%(SDS-PAGE)

表达系统

E. coli

种属

人(Human)

氨基酸

1-261 aa

序列

MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRDGFKLVMASLYHIYVALEEEIERNKESPVFAPVYFPEELHRKAALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQVLKKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNSLEMTPAVRQRVIEEAKTAFLLNIQLFEELQELLTHDTKDQSPSRAPGLRQRASNKVQDSAPVETPRGKPPLNTHHHHHH

纯度

>98% (SDS-PAGE)

蛋白标签

C-His

无载体

Yes

无动物源

来源

重组表达

预测分子量

30.7 kDa

SDS-PAGE

28.8 kDa, reducing conditions; 28.8 kDa, non-reducing conditions

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from 20mM Tris, 400mM NaCl, pH8.0

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。

图片

SDS-PAGE: Recombinant Human Heme Oxygenase 1 Protein (rp176715)

Recombinant Human Heme Oxygenase 1 Protein (rp176715) - SDS-PAGE
Recombinant Human Heme Oxygenase 1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.8 kDa.