级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥95%(SDS-PAGE)See COA
Accession #
O75874-1
蛋白标签
C-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
1、Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions. 2、Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC50 of 11.16 ng/mL.
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),See COA
生化机理
Isocitrate Dehydrogenase 1 (IDH1) catalyzes the oxidative decarboxylation of isocitrate to alpha ‑ketoglutarate. There are two subclasses in the IDH family, one of them utilizing NADP+ as the electron acceptor and the other using NAD+. The protein encoded by this gene is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. In peroxisomes, IDH1 generates the NADPH required for intraperoxisomal reduction reactions. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha ‑ketoglutarate. Elevated levels of the metabolite 2HG are associated with a high risk of malignant brain tumors. Arg132 mutations of IDH1 are common in high‑grade gliomas, but not in other types of tumors.
Post-translational: Acetylation at Lys-374 dramatically reduces catalytic activity.
生物活性
1、Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions. 2、Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC50 of 11.16 ng/mL.
44.2 kDa, under reducing conditions; 43.6 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
50 mM Tris, 150 mM NaCl, 1 mM TCEP, 30% Glycerol, pH7.5
浓度
See COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) - SDS-PAGE 3 μg/lane of Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 44.2 kDa under reducing conditions and 43.6 kDa under non-reducing conditions.
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein - Enzyme Activity Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10,000 pmol/min/μg, as measured under the described conditions. Materials: Assay Buffer: 25 mM Tris, 0.5 mM MnCl₂, 5 mM DTT, pH 7.5 Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) DL-Isocitric acid (I106830), 100 mM stock in deionized water NADP⁺ (N303921), 50 mM stock in deionized water
Procedures： 1. Dilute rhIDH1 to 0.4 ng/μL in Assay Buffer. 2. Prepare a Substrate Mixture by Diluting NADP
⁺
and Isocitric Acid to 1 mM and 2 mM, respectively, in Assay Buffer. 3. Load into a plate 50 μL of 0.4 ng/μL rhIDH1 and start the reaction by adding 50 μL of Substrate Mix. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of Substrate Mix. 4. Read plate at a wavelength of 340 nm (bottom read) in kinetic mode for 5 minutes. 5. Calculate specific activity: *Adjusted for Substrate Blank **Using the extinction coefficient 6270 M⁻¹cm⁻¹ ***Using the path correction 0.32 cm Per Well: rhIDH1: 0.020 μg, NADP⁺
: 0.5 mM, Isocitric Acid: 1 mM
Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein - ELISA Immobilized Recombinant Human Isocitrate Dehydrogenase 1/IDH1 Protein (rp179799) at 3.0 μg/mL IDH1 Mouse mAb (Ab109324) with the EC₅₀ of 11.16 ng/mL.
*Adjusted for Substrate Blank
ZJ25F0420338
危险品化学品经营许可证（带存储）