Immobilized Recombinant Human Procalcitonin Protein (rp179804) at 1.0 μg/mL can bind Procalcitonin (PCT) Mouse mAb (Ab008053) with the EC50 of 6.3 ng/mL.
Procalcitonin (PCT) is encoded by a single CALCA gene, and can result in two alternatively spliced products: calcitonin and calcitonin-gene related peptide (CGRP). Mature PCT is expressed as a 116 amino acid (aa) protein which is subsequently cleaved into three parts: a 57 aa pro-region, a 32 aa Calcitonin peptide, and a 21 aa Katacalcin peptide. PCT is moderately conserved among species, with human sharing 72% and 75% sequence identity with mouse and rat, respectively. In healthy adults, PCT is expressed by thyroid C cells and promptly converted to calcitonin. Calcitonin is directly involved in osteoclast activity and bone resorption. However, in states of inflammatory disease or bacterial infection, PCT is over-expressed from multiple cell types and the levels of unprocessed PCT rise significantly. These increased levels of PCT in circulation have been used as a diagnostic marker of systemic bacterial infection and sepsis and as a guide to antibiotic therapy. More recently, the use of PCT as a disease marker has been expanded to include acute heart failure and vasculitic disorders.
生物活性
Immobilized Recombinant Human Procalcitonin Protein (rp179804) at 1.0 μg/mL can bind Procalcitonin (PCT) Mouse mAb (Ab008053) with the EC50 of 6.3 ng/mL.
12.6 kDa, under reducing conditions; 12.8 & 27.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Procalcitonin Protein (rp179804) - ELISA Immobilized Recombinant Human Procalcitonin Protein (rp179804) at 1.0 μg/mL can bind Procalcitonin (PCT) Mouse mAb (Ab008053) with the EC₅₀ of 6.3 ng/mL.
Recombinant Human Procalcitonin Protein (rp179804) - SDS-PAGE 3 μg/lane of Recombinant Human Procalcitonin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.6 kDa under reducing conditions and 12.8 & 27.4 kDa under non-reducing conditions. Under certain specific conditions, Procalcitonin has been suggested to have the capacity to form Dimer (PMID: 32615866).