Immobilized Recombinant Human MMP-14/MT1-MMP Protein (rp181212) at 1.0 μg/mL can bind Recombinant MMP14 Antibody (Ab115644) with the EC50 of 8.7 ng/mL.
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V. MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail. Recombinant Human MMP-14 consists of the pro and catalytic domains, which can be activated by treatment with furin as described in the Activity Assay Protocol.
Post-translational: The precursor is cleaved by a furin endopeptidase.Tyrosine phosphorylated by PKDCC/VLK.
生物活性
Immobilized Recombinant Human MMP-14/MT1-MMP Protein (rp181212) at 1.0 μg/mL can bind Recombinant MMP14 Antibody (Ab115644) with the EC50 of 8.7 ng/mL.
56.9-64.6 kDa, under reducing conditions; 53.0-60.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 5% Glycerol, pH 7.4
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human MMP-14/MT1-MMP Protein (rp181212) - ELISA Immobilized Recombinant Human MMP-14/MT1-MMP Protein (rp181212) at 1.0 μg/mL can bind Recombinant MMP14 Antibody (Ab115644) with the EC50 of 8.7 ng/mL.
Recombinant Human MMP-14/MT1-MMP Protein (rp181212) - SDS-PAGE 3 μg/lane of Recombinant Human MMP-14/MT1-MMP Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56.9-64.6 kDa under reducing conditions and 53.0-60.2 kDa under non-reducing conditions.