计算溶液所需的质量、体积或浓度。
Recombinant Human Dkk-1 Protein (rp181236) - ELISA
Immobilized Recombinant Human Dkk-1 Protein (rp181236) at 1.0 μg/mL can bind Recombinant DKK1 Antibody (Ab099940) with the EC₅₀ of 5.57 ng/mL.
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Protein Function:
Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues. Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90%, and 91% aa sequence identity with mouse, rat, rabbit, and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well-documented antagonists of the canonical Wnt signaling pathway. This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6. Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2. Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling. Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. Other sites of expression include developing neurons, hair follicles, and the retina of the eye. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis. Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively. In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death.
Recombinant Human Dkk-1 Protein (rp181236) - ELISA
Immobilized Recombinant Human Dkk-1 Protein (rp181236) at 1.0 μg/mL can bind Recombinant DKK1 Antibody (Ab099940) with the EC₅₀ of 5.57 ng/mL.
Recombinant Human Dkk-1 Protein (rp181236) - SDS-PAGE
3 μg/lane of Recombinant Human Dkk-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 37.3-47.6 kDa under reducing conditions and 42.4-60.6 kDa under non-reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp181236 | |
| 分析证书 | rp181236 | |
| 分析证书 | rp181236 | |
| 分析证书 | rp181236 |
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