Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG). Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors. Gas6 induces a variety of responses, including prevention of apoptosis, cell proliferation, platelet-mediated thrombosis, retinal epithelial cell phagocytosis of outer rod segments, inhibition of VEGF-induced endothelial cell chemotaxis, and the differentiation and expansion of NK cell precursors. The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells. Several of these effects have been shown to require gamma -carboxylation of the Gla domain.
Post-translational: Isoform 2. Proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
51.7 & 56.9 & 67.2 kDa, under reducing conditions; 55.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20℃ for 12 months.Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.
图片
Recombinant Human Gas6 Protein (rp181243) - SDS-PAGE 3 μg/lane of Recombinant Human Gas6 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 51.7 & 56.9 & 67.2 kDa under reducing conditions and 55.7 kDa under non-reducing conditions. Under reducing conditions, disulfide bonds are disrupted, potentially leading to more pronounced protein degradation. Additionally, the 49-678aa fragment of Gas6 may retain partial γ-carboxylation (Gla domain modification), and post-translational modifications could result in slight band diffusion (Uniprot ID: Q14393).
ZJ25F0826012
危险品化学品经营许可证（带存储）