Measured by the ability of the immobilized protein to support the adhesion of 5 μg/mL PHA-stimulated human Jurkat T lymphocyte leukemia cell for 2h. The ED50 for this effect is 0.1892 μg/mL.
Protein Function: Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity.
规格
产品名称
Recombinant Human ICAM-1/CD54 Protein
别名
重组人ICAM-1/CD54蛋白 | 重组人类 ICAM-1/CD54 蛋白
英文别名
CD54 antigen | CD54 | cell surface glycoprotein P3.58 | human rhinovirus receptor | ICAM1 | ICAM-1 | intercellular adhesion molecule 1 (CD54), human rhinovirus receptor | intercellular adhesion molecule 1 | Major group rhinovirus receptor | P3.58 | BB2
Measured by the ability of the immobilized protein to support the adhesion of 5 μg/mL PHA-stimulated human Jurkat T lymphocyte leukemia cell for 2h. The ED50 for this effect is 0.1892 μg/mL.
62.6-88.8 kDa, under reducing conditions; 58.1-81.5 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human ICAM-1/CD54 Protein (rp181245) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of 5 μg/mL PHA-stimulated human Jurkat T lymphocyte leukemia cell for 2h. The ED₅₀ for this effect is 0.1892 μg/mL.
Recombinant Human ICAM-1/CD54 Protein (rp181245) - SDS-PAGE 3 μg/lane of Recombinant Human ICAM-1/CD54 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 62.6-88.8 kDa under reducing conditions and 58.1-81.5 kDa under non-reducing conditions.
ZJ24F0707696
危险品化学品经营许可证（带存储）