级别和纯度: 无动物源?无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥90%(SDS-PAGE)expressed in HEK293; 见COA
Accession #
Q9NPF7
蛋白标签
C-hFc
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human IL-23 P19/IL23A Protein (rp181249) at 1.0 μg/mL can bind Mirikizumab (anti-IL-23) (Ab175599) with the EC50 of 931.6 ng/mL.
无动物源,无载体,生物活性,ActiBioPure™,Fc标签,≥90%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
Associates with IL12B to form the pro-inflammatory cytokine IL-23 that plays different roles in innate and adaptive immunity (PubMed:11114383).
Released by antigen-presenting cells such as dendritic cells or macrophages, binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R to activate JAK2 and TYK2 which then phosphorylate the receptor to form a docking site leading to the phosphorylation of STAT3 and STAT4 (PubMed:29287995, PubMed:32474165, PubMed:33606986).
This process leads to activation of several pathways including p38 MAPK or NF-kappa-B and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A
生物活性
Immobilized Recombinant Human IL-23 P19/IL23A Protein (rp181249) at 1.0 μg/mL can bind Mirikizumab (anti-IL-23) (Ab175599) with the EC50 of 931.6 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human IL-23 P19/IL23A Protein (rp181249) - ELISA
Immobilized Recombinant Human IL-23 P19/IL23A Protein (rp181249) at 1.0 μg/mL can bind Mirikizumab (anti-IL-23) (Ab175599) with the EC50 of 931.6 ng/mL.
Recombinant Human IL-23 P19/IL23A Protein (rp181249) - SDS-PAGE
3 μg/lane of Recombinant Human IL-23 P19/IL23A Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 44.5-50.7 kDa under reducing conditions and 96.6 kDa under non-reducing conditions.
There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID: Q9NPF7).