Recombinant Human Insulin R/CD220 Protein

Name: Recombinant Human Insulin R/CD220 Protein
SKU: rp181255
Availability: InStock

货号: rp181255

COA

有货

级别和纯度: 无动物源 ? 无载体 ? 生物活性 ? ActiBioPure™ ? His标签 ? PBS Only ? ≥95%(SDS-PAGE) 见COA

Accession #

P06213-2

蛋白标签

C-10His

表达系统

CHO

内毒素水平

<1.0 EU/μg

生物活性

Immobilized Insulin R/CD220 (aa 28-944) (rp181255) at 1.0 μg/mL can bind Recombinant Insulin Receptor Antibody (Ab110735) with the EC50 of 6.3 ng/mL.

规格

库存

价格

数量

10μg

rp181255-10μg

现货

¥799.90

50μg

rp181255-50μg

现货

¥1,999.90

100μg

rp181255-100μg

现货

¥3,139.90

1mg

rp181255-1mg

期货

¥16,999.90

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Recombinant Human Insulin R/CD220 Protein

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规格储存与运输图片技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器常见问题

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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无动物源,无载体,生物活性级提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称

Recombinant Human Insulin R/CD220 Protein

别名

重组人Insulin R/CD220蛋白 | 重组人胰岛素R/CD220蛋白

英文别名

CD 220 | CD220 antigen | CD220 | EC 2.7.10 | EC 2.7.10.1 | HHF5 | INSR | Insulin R | insulin receptor | InsulinR | IR

规格或纯度

无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥95%(SDS-PAGE),见COA

生化机理

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. Post-translational: After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. Autophosphorylated on tyrosine residues in response to insulin (PubMed:14690593, PubMed:16246733, PubMed:16271887, PubMed:18278056, PubMed:18767165, PubMed:3166375, PubMed:9312016). Phosphorylation of Tyr-999 is required for binding to IRS1, SHC1 and STAT5B (PubMed:14690593, PubMed:16246733, PubMed:16271887, PubMed:18278056, PubMed:18767165, PubMed:3166375, PubMed:9312016). Dephosphorylated by PTPRE at Tyr-999, Tyr-1185, Tyr-1189 and Tyr-1190 (PubMed:14690593, PubMed:16246733, PubMed:16271887, PubMed:18278056, PubMed:18767165, PubMed:3166375, PubMed:9312016). May also be phosphorylated at Tyr-1185 and Tyr-1190 by mTORC2 (PubMed:26584640). Dephosphorylated by PTPRF and PTPN1 (PubMed:8995282). Dephosphorylated by PTPN2; down-regulates insulin-induced signaling (PubMed:10734133, PubMed:12612081). Dephosphorylation at Tyr-1189 and Tyr-1190 requires the SH2/SH3 adapter protein NCK1, probably to recruit its interaction partner PTPN1 (PubMed:21707536). S-nitrosylation at Cys-1083 by BLVRB inhibits the receptor tyrosine kinase, thereby inhibiting insulin signaling. Ubiquitinated by MARCHF1; leading to degradation thereby reducing surface INSR expression.

生物活性

Immobilized Insulin R/CD220 (aa 28-944) (rp181255) at 1.0 μg/mL can bind Recombinant Insulin Receptor Antibody (Ab110735) with the EC50 of 6.3 ng/mL.

表达系统

CHO

种属

人(Human)

氨基酸

1-956 aa

序列

HLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGPCPKVCHLLEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSYIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLRSNDPKSQNHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVFWERQAEDSELFELDYCLKGLKLPSRTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRKTSSGTGAEDPRPSRKRRSLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKHHHHHHHHHH

蛋白标签

C-10His

无载体

Yes

无动物源

Yes

来源

重组表达

预测分子量

107.2 kDa

SDS-PAGE

36.3 & 41.0 & 90.0 & 119.6 kDa, under reducing conditions; >250 kDa, under non-reducing conditions.

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4.

浓度

见COA

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

图片

ELISA: Recombinant Human Insulin R/CD220 (rp181255)

Recombinant Human Insulin RCD220 (rp181255) - ELISA
Immobilized Insulin R/CD220 (rp181255) at 1.0 μg/mL can bind Recombinant Insulin Receptor Antibody (Ab110735) with the EC50 of 6.3 ng/mL.

SDS-PAGE: Recombinant Human Insulin R/CD220 Protein (rp181255)

Recombinant Human Insulin R/CD220 Protein (rp181255) - SDS-PAGE
3 μg/lane of Recombinant Human Insulin R/CD220 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 36.3 & 41.0 & 90.0 & 111.2-128.0 kDa under reducing conditions and >250.0 kDa under non-reducing conditions.
The observation of Insulin R/CD220 Protein as both dimers and monomers under non-reducing (NR) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds. Under reducing conditions, disulfide bonds are disrupted, potentially leading to more pronounced protein degradation (Uniprot: P06213-2).

技术文档

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0825754	分析证书	rp181255
ZJ25F0825755	分析证书	rp181255
ZJ25F0825756	分析证书	rp181255

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计算配制储备溶液所需的稀释度。

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体积（添加到小瓶中）

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本产品如何运输？

本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。

本产品的纯度是多少？如何测定？

本产品的纯度为 ≥95%，采用SDS-PAGE测定。批次的具体数值以分析证书（COA）为准。

什么是「无动物源」？

「无动物源」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

什么是「无载体」？

「无载体」表示该物料经过加工和检测，适用于特种工业加工。规格侧重于影响该应用性能的杂质——例如，电子应用的金属含量、电池应用的粒度、光学应用的折射率，或对水分敏感工艺的水分含量。

本产品应如何储存？

请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。

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Recombinant Human Insulin R/CD220 Protein

储存与运输

质量文档

文献证明

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

常见问题

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