Recombinant Human TGF-beta 3 Protein

Name: Recombinant Human TGF-beta 3 Protein
SKU: rp181280
Availability: InStock

货号: rp181280

COA

有货

级别和纯度: 无动物源 ? 无载体 ? 生物活性 ? ActiBioPure™ ? 高性能 ? His标签 ? ≥90%(SDS-PAGE) expressed in HEK293; 见COA

Accession #

P10600

蛋白标签

C-His

表达系统

HEK293

内毒素水平

<1.0 EU/μg

生物活性

Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 2.3 pg/mL.

规格

库存

价格

数量

10μg

rp181280-10μg

期货

¥999.90

50μg

rp181280-50μg

期货

¥2,999.90

100μg

rp181280-100μg

期货

¥4,799.90

1mg

rp181280-1mg

期货

¥22,999.90

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规格储存与运输图片技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 溶液计算器

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为什么选择此级别

ActiBioPure™,His标签,无动物源,无载体,生物活性,高性能级提供 ≥90%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称

Recombinant Human TGF-beta 3 Protein

别名

重组人TGF-beta 3蛋白 | 重组人转化生长因子β3蛋白

英文别名

规格或纯度

无动物源, 无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥90%(SDS-PAGE), expressed in HEK293; 见COA

生化机理

TGF-beta 3 (transforming growth factor-beta 3) is a member of a TGF-beta superfamily subgroup that is defined by their structural and functional similarities. TGF-beta 3 and its closely related proteins, TGF-beta 1 and beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial-mesenchymal transition. The non-redundant biological effects of TGF- beta 3 include involvement in palatogenesis, chondrogenesis, and pulmonary development. Human TGF-beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 20 aa signal peptide and a 392 aa proprotein. The proprotein is processed by a furin-like convertase to generate a 220 aa latency-associated peptide (LAP) and a 112 aa mature TGF-beta 3. Mature human TGF- beta 3 shows 100%, 99%, and 98% aa identity with mouse/dog/horse, rat, and pig TGF- beta 3, respectively. TGF-beta 3 is secreted as a latent complex. This latent form of TGF-beta 3 is activated by integrins, thrombospondin-1, plasmin, and matrix metalloproteases. It can also be activated by extreme pH and reactive oxygen species. TGF-beta 3 binds with high affinity to TGF-beta RII, a type II serine/threonine kinase receptor. This receptor then phosphorylates and activates type I serine/threonine kinase receptors, TGF- beta RI or ALK-1, to modulate transcription through Smad phosphorylation. The divergent biological effects exerted by individual TGF-beta isoforms is dependent upon the recruitment of co-receptors (TGF- beta RIII and endoglin) and the subsequent initiation of Smad-dependent or -independent signaling pathways. Post-translational: Transforming growth factor beta-3 proprotein: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-3 (TGF-beta-3) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-3 inactive. Methylated at Gln-293 by N6AMT1.

生物活性

Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 2.3 pg/mL.

表达系统

HEK293

种属

人(Human)

氨基酸

1-412 aa

序列

MKMHLQRALVVLALLNFATVSLSLSTCTTLDFGHIKKKRVEAIRGQILSKLRLTSPPEPTVMTHVPYQVLALYNSTRELLEEMHGEREEGCTQENTESEYYAKEIHKFDMIQGLAEHNELAVCPKGITSKVFRFNVSSVEKNRTNLFRAEFRVLRVPNPSSKRNEQRIELFQILRPDEHIAKQRYIGGKNLPTRGTAEWLSFDVTDTVREWLLRRESNLGLEISIHCPCHTFQPNGDILENIHEVMEIKFKGVDNEDDHGRGDLGRLKKQKDHHNPHLILMMIPPHRLDNPGQGGQRKKRALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCSHHHHHH

蛋白标签

C-His

无载体

Yes

无动物源

Yes

来源

重组表达

预测分子量

45.6 kDa

SDS-PAGE

12.0-15.0 & 39.3 & 51.8 kDa, under reducing conditions; 49.3 & 95.0-109.0 kDa, under non-reducing conditions.

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4

浓度

expressed in HEK293; 见COA

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

-20℃保存 12个月；复溶后建议分装，避免反复冻融。

图片

Protein Bioactivity: Recombinant Human TGF-beta 3 Protein (rp181280)

Recombinant Human TGF-beta 3 Protein (rp181280) - Protein Bioactivity

Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED₅₀ for this effect is 2.3 pg/mL.

SDS-PAGE: Recombinant Human TGF-beta 3 Protein (rp181280)

Recombinant Human TGF-beta 3 Protein (rp181280) - SDS-PAGE

3 μg/lane of Recombinant Human TGF-beta 3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 12.0-15.0 & 39.3 & 51.8 kDa under reducing conditions and 49.3 & 95.0-109.0 kDa under non-reducing conditions. Transforming growth factor β-3 (TGF-beta 3) is a homodimer connected by disulfide bonds (PubMed: 8819159).

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

查看数据表 →

🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

需要帮助选择级别？

我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。

查看 Animal Free 级别指南 → 查看 Carrier Free 级别指南 → 查看 Bioactive 级别指南 → 查看 ActiBioPure™ 级别指南 → 查看 High Performance 级别指南 → 查看 His Tag 级别指南 →

大包装与定制包装 需要更大包装或定制规格？请联系我们的团队获取专属报价。

Recombinant Human TGF-beta 3 Protein

储存与运输

质量文档

文献证明

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

需要帮助选择级别？

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