级别和纯度: 无动物源?无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。无载体?无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。生物活性?生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc标签?Fc 标签级 —— 带有 Fc 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。Fc 标签有助于二聚化和 protein A/G 捕获。≥95%(SDS-PAGE)见COA
Accession #
P23510
蛋白标签
N-hFc & His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human OX40 Ligand/TNFSF4 Protein (rp181288) at 1.0 μg/mL can bind Amlitelimab (anti-TNFSF4) (Ab190156) with the EC50 of 49.2 ng/mL.
OX-40L, also known as TNFSF4 and CD252, is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. OX-40L is an important costimulatory molecule that plays a crucial role in the regulation of T-cell-mediated immunity. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. OX-40L is a ligand for receptor TNFRSF4/OX40. It is found to play a role in T cell antigen-presenting cell (APC) interactions. In surface Ig- and CD4-stimulated B cells, this cytokine along with CD7 has been shown to provide CD28-independent costimulatory signals to T cells. This protein and its receptor are reported to directly mediate adhesion of activated T cells to vascular endothelial cells.
生物活性
Immobilized Recombinant Human OX40 Ligand/TNFSF4 Protein (rp181288) at 1.0 μg/mL can bind Amlitelimab (anti-TNFSF4) (Ab190156) with the EC50 of 49.2 ng/mL.
49.5-56.2 kDa, under reducing conditions; 94.1-108.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human OX40 Ligand/TNFSF4 Protein (rp181288) - ELISA
Immobilized Recombinant Human OX40 Ligand/TNFSF4 Protein (rp181288) at 1.0 μg/mL can bind Amlitelimab (anti-TNFSF4) (Ab190156) with the EC₅₀ of 49.2 ng/mL.
Recombinant Human OX40 Ligand/TNFSF4 Protein (rp181288) - SDS-PAGE
3 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 49.5-56.2 kDa under reducing conditions and 94.1-108.4 kDa under non-reducing conditions.
Fc fusion proteins can be linked by disulfide bonds in the Fc hinge region to form stable dimers.(PMID: 23665380)