计算溶液所需的质量、体积或浓度。
Recombinant Human VISTA/B7-H5 Protein (rp181308) - ELISA
Immobilized Recombinant Human VISTA/B7-H5 Protein (rp181308) at 1.0 μg/mL can bind Onvatilimab (anti-VISTA) (Ab170685) with the EC₅₀ of 48.93 ng/mL.
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Protein Function:
Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules. Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively. The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane. Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2. Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure. It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4. Gi24 is expressed on the surface of naïve CD4+ T cells, regulatory T cells, and adipocytes. It is up-regulated in vivo on activated monocytes and dendritic cells. Gi24 inhibits CD4+ and CD8+ T cell proliferation, and their production of IL-2 and IFN-gamma. Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression. In contrast, Gi24 limits disease progression in the autoimmune disease model EAE. Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance.
Recombinant Human VISTA/B7-H5 Protein (rp181308) - ELISA
Immobilized Recombinant Human VISTA/B7-H5 Protein (rp181308) at 1.0 μg/mL can bind Onvatilimab (anti-VISTA) (Ab170685) with the EC₅₀ of 48.93 ng/mL.
Recombinant Human VISTA/B7-H5 Protein (rp181308) - SDS-PAGE
3 μg/lane of Recombinant Human VISTA/B7-H5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 33.0-46.3 kDa under reducing conditions and 31.3-43.6 kDa under non-reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp181308 | |
| 分析证书 | rp181308 | |
| 分析证书 | rp181308 | |
| 分析证书 | rp181308 |
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