Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. In addition to be a subunit of the proinflammatory cytokine IL-12, p35 is also a subunit of IL-35, an immune suppressor. p35 subunit is broadly expressed while p40 subunit is limited to hematopoietic cells. Secretion of p35 is dependent on p40 to ensure correct folding. Recent studies show that p40 and p35 which are expressed from two different cell lines can assemble to form functional IL-12 in vitro. When functions alone, p35 subunit induces expansion of IL-10 and IL-35 expressing regulatory B cells and inhibits neuroinflammation and ameliorates autoimmune encephalomyelitis.
58.1-70.7 kDa, under reducing conditions; 112.4-123.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IL-12 p35 (C96S) Protein (rp181557) - SDS-PAGE 2 μg/lane of Recombinant Human IL-12 p35 (C96S) Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 58.1-70.7 kDa under reducing conditions and 112.4-123.7 kDa under non-reducing conditions.
ZJ25F0724435
危险品化学品经营许可证（带存储）