Immobilized Recombinant Human EphA2 Protein (rp181601) at 2.0 μg/mL can bind Recombinant Human Ephrin-A4 Protein (rp145636) with the EC50 of 7.414 ng/mL.
Protein Function: Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.
Immobilized Recombinant Human EphA2 Protein (rp181601) at 2.0 μg/mL can bind Recombinant Human Ephrin-A4 Protein (rp145636) with the EC50 of 7.414 ng/mL.
60.4 kDa, under reducing conditions; 50.5 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human EphA2 Protein (rp181601) - Binding Activity Immobilized Recombinant Human EphA2 Protein (rp181601) at 2.0 μg/mL can bind Recombinant Human Ephrin-A4 Protein (rp145636) with the EC
₅₀
of 7.414 ng/mL.
Recombinant Human EphA2 Protein (rp181601) - SDS-PAGE 3 μg/lane of Recombinant Human EphA2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 60.4 kDa under reducing conditions and 50.5 kDa under non-reducing conditions.
ZJ25F0825311
危险品化学品经营许可证（带存储）