计算溶液所需的质量、体积或浓度。
Recombinant Human Lumican Protein (rp181608) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of A375 human melanoma cells. The ED
₅₀
for this effect is 1.01 μg/mL.
ActiBioPure™,His标签,PBS Only,无动物源,无载体,生物活性,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily. Human Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain. The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines. Mature human Lumican is 88%, 87%, and 70% aa identical to mature mouse, rat, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins. Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage. Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers. The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis. In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation, migration, and adhesion. Lumican’s over-expression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers.
Recombinant Human Lumican Protein (rp181608) - Protein Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of A375 human melanoma cells. The ED
₅₀
for this effect is 1.01 μg/mL.
Recombinant Human Lumican Protein (rp181608) - SDS-PAGE
3 μg/lane of Recombinant Human Lumican Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 44.2-54.7 kDa under reducing conditions and 44.0-54.5 kDa under non-reducing conditions.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | rp181608 | |
| 分析证书 | rp181608 | |
| 分析证书 | rp181608 | |
| 分析证书 | rp181608 |
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