Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
生物活性
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.
61.1 kDa, under reducing conditions; 61.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM PB, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - ELISA Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC₅₀ of 13.1 ng/mL.
Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - ELISA Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC₅₀ of 2.1 ng/mL.
Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - SDS-PAGE 1 μg/lane of Recombinant Human 5'-nucleotidase/CD73 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 61.1 kDa under reducing conditions and 61.7 kDa under non-reducing conditions.