Recombinant Human 5'-nucleotidase/CD73 Protein

货号: rp181611
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥95%(SDS-PAGE) 见COA
Accession #
P21589-1
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.
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规格
库存
价格
数量
10μg
rp181611-10μg
现货 Stock Image
¥599.90
50μg
rp181611-50μg
现货 Stock Image
¥1,999.90
100μg
rp181611-100μg
现货 Stock Image
¥3,299.90
1mg
rp181611-1mg
期货 Stock Image
¥15,399.90
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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无动物源,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称
Recombinant Human 5'-nucleotidase/CD73 Protein
别名
重组人5'-nucleotidase/CD73蛋白 | 重组人跨膜糖蛋白
英文别名
5' nucleotidase (CD73) | 5'-NT | 5-NT | 5'-Nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 antigen | CD73 | E5NT | EC 3.1.3.5 | ecto-5'-nucleotidase | eN | eNT | NT | NT55'-nucleotidase | NT5E | NTE | Purine 5-Prime-Nucleotidase
规格或纯度
无动物源,无载体,生物活性,ActiBioPure™,His标签,PBS Only,≥95%(SDS-PAGE),见COA
生化机理
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
生物活性
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC50 of 13.1 ng/mL. Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC50 of 2.1 ng/mL.
表达系统
HEK293
种属
人(Human)
氨基酸
1-547 aa
序列
MCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIKHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
58.6 kDa
SDS-PAGE
61.1 kDa, under reducing conditions; 61.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM PB, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片

Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - ELISA
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Mupadolimab (anti-CD73) (Ab170487) with the EC₅₀ of 13.1 ng/mL.


Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - ELISA
Immobilized Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) at 1.0 μg/mL can bind Oleclumab (anti-CD73) (Ab170578) with the EC₅₀ of 2.1 ng/mL.

Recombinant Human 5'-nucleotidase/CD73 Protein (rp181611) - SDS-PAGE
1 μg/lane of Recombinant Human 5'-nucleotidase/CD73 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 61.1 kDa under reducing conditions and 61.7 kDa under non-reducing conditions.

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

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📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ25F0217973 分析证书 rp181611
ZJ25F0217974 分析证书 rp181611
ZJ25F0217975 分析证书 rp181611
技术文档和文章
溶液计算器