Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha, amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF family members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region. BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotein. The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing. Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively. The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5. BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum. BTC promotes pancreatic beta-cell growth and differentiation and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts. The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers.
生物活性
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1.41 ng/mL.
13.3 kDa, under reducing conditions; 14.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Mouse Betacellulin Protein (rp183570) - Bioactivity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1.41 ng/mL.
Recombinant Mouse Betacellulin Protein (rp183570) - SDS-PAGE 3 μg/lane of Recombinant Mouse Betacellulin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.3 kDa under reducing conditions and 14.7 kDa under non-reducing conditions.