CCL16, a chemokine poorly characterized at the functional level. Human CCL16 is a member of the CC family, and its gene maps to human chromosome 17q. In the mouse, only a pseudogene has been identified to date. CCL16 is a functional ligand for CCR1, CCR2, CCR5, and CCR8. Recombinant CCL16 demonstrated chemotactic activity on human monocytes and lymphocytes. Based on the ability of human chemokines to exert activity on and bind to murine receptors, the TSA mouse adenocarcinoma cell line was transfected with human CCL16 cDNA and, in comparison with other cytokines, was shown to be the faster inducer of systemic immune response due to massive, prompt infiltration of leukocytes.
生物活性
Determined by its ability to chemoattract total human monocytes using a concentration range of 10.0-100.0 ng/ml.
12.1 kDa, under reducing conditions; 12.1-17.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human CCL16/HCC-4 Protein (rp183575) - Protein Bioactivity
Determined by its ability to chemoattract total human monocytes using a concentration range of 10.0-100.0 ng/mL.
Recombinant Human CCL16/HCC-4 Protein (rp183575) - SDS-PAGE
3 μg/lane of Recombinant Human CCL16/HCC-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 12.1 kDa under reducing conditions and 12.1-17.0 kDa under non-reducing conditions.
There are multiple pairs of disulfide bonds in the protein sequence. Disulfide bonds cause the protein to present as a polymer in a non-reducing conditions (Uniprot ID: O15467).