1. Immobilized Recombinant Human S100A8 Protein (rp183594) at 2.0 μg/mL can bind Recombinant Human S100A9 Protein (rp183637) with the EC50 of 135.5 ng/mL. 2. Immobilized Recombinant Human S100A9 Protein (rp183637) at 2.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC50 of 194.6 ng/mL. 3. Immobilized Recombinant Human S100A8 Protein (rp183594) at 1.0 μg/mL can bind S100A8 Mouse mAb (Ab182004) with the EC50 of 18.63 ng/mL.
Protein Function: S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium-binding proteins. They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid. The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF-hand calcium-binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively. S100A8 and S100A9 are noncovalent homodimers that can also noncovalently heterodimerize; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers. The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria. The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation. S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes.
规格
产品名称
Recombinant Human S100A8 Protein
别名
重组人S100A8蛋白 | 重组人S100A8蛋白
英文别名
Calprotectin L1L subunit | Cystic fibrosis antigen (CFAG) | Leukocyte L1 complex light chain | Migration inhibitory factor-related protein 8 (MRP-8 | p8) | S100 calcium-binding protein A8 | Urinary stone protein band A | Calgranulin-A
规格或纯度
无载体, 生物活性, ActiBioPure™, His标签, ≥95%(SDS-PAGE)
生物活性
1. Immobilized Recombinant Human S100A8 Protein (rp183594) at 2.0 μg/mL can bind Recombinant Human S100A9 Protein (rp183637) with the EC50 of 135.5 ng/mL. 2. Immobilized Recombinant Human S100A9 Protein (rp183637) at 2.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC50 of 194.6 ng/mL. 3. Immobilized Recombinant Human S100A8 Protein (rp183594) at 1.0 μg/mL can bind S100A8 Mouse mAb (Ab182004) with the EC50 of 18.63 ng/mL.
11.7 kDa, under reducing conditions; 11.7 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 1mM DTT, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human S100A8 Protein (rp183594) - Binding Activity Immobilized Recombinant Human S100A8 Protein (rp183594) at 2.0 μg/mL can bind Recombinant Human S100A9 Protein (rp183637) with the EC
₅₀
of 135.5 ng/mL.
Recombinant Human S100A8 Protein (rp183594) - Binding Activity Immobilized Recombinant Human S100A9 Protein (rp183637) at 2.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC
₅₀
of 194.6 ng/mL.
Recombinant Human S100A8 Protein (rp183594) - ELISA Immobilized Recombinant Human S100A8 Protein (rp183594) at 1.0 μg/mL can bind S100A8 Mouse mAb (Ab182004) with the EC
₅₀
of 18.63 ng/mL.
Recombinant Human S100A8 Protein (rp183594) - SDS-PAGE 2 μg/lane of Recombinant Human S100A8 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 11.7 kDa.
ZJ24F0707737
危险品化学品经营许可证（带存储）