Recombinant Human EphA4 Protein

货号: rp183616
有货
级别和纯度: 无动物源 ? 无动物源(Animal Free)—— 不含动物源成分生产,降低污染风险。适用于避免动物源材料的生物制造和培养。 无载体 ? 无载体(Carrier Free)—— 不含添加的载体蛋白/稳定剂。当载体(如 BSA)会干扰偶联或敏感检测时使用。 生物活性 ? 生物活性级(Bioactive)—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。 ActiBioPure™ ? ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。 His标签 ? 组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白,供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。 PBS Only ? 仅 PBS 配方 —— 以磷酸盐缓冲液供应,不含其他添加剂。当需要不含稳定剂/载体的洁净 PBS 缓冲液时使用。 ≥95%(SDS-PAGE) 见COA
Accession #
P54764
蛋白标签
C-His
表达系统
HEK293
内毒素水平
<1.0 EU/μg
生物活性
Immobilized Recombinant Human EphA4 Protein (rp183616) at 2.0 μg/mL can bind Human Recombinant Ephrin-A4 Protein (rp145636) with the EC50 of 8.848 ng/mL.
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规格
库存
价格
数量
10μg
rp183616-10μg
现货 Stock Image
¥699.90
50μg
rp183616-50μg
现货 Stock Image
¥1,899.90
100μg
rp183616-100μg
现货 Stock Image
¥2,849.90
1mg
rp183616-1mg
期货 Stock Image
¥12,159.90
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为什么选择此级别

ActiBioPure™,His标签,PBS Only,无动物源,无载体,生物活性 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

概述


Protein Function:
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity).
Phosphorylates CAPRIN1, promoting CAPRIN1-dependent formation of a membraneless compartment (By similarity).

规格

产品名称
Recombinant Human EphA4 Protein
别名
重组人EphA4蛋白 | 重组人酪氨酸蛋白激酶受体A4蛋白 | 重组人EphA4蛋白
英文别名
CEK8 | Cek 8 | EK8 | eph receptor a4 | EPH-like kinase 8 | EPHA4 | EPHA4_HUMAN | Ephrin type-A receptor 4 | HEK 8 | hEK8 | Receptor protein-tyrosine kinase HEK8 | SEK | Sek 1 | TYRO 1 protein tyrosine kinase | TYRO1 | Tyrosine-protein kinase receptor SEK
规格或纯度
无动物源, 无载体, 生物活性, ActiBioPure™, His标签, PBS Only, ≥95%(SDS-PAGE), 见COA
生物活性
Immobilized Recombinant Human EphA4 Protein (rp183616) at 2.0 μg/mL can bind Human Recombinant Ephrin-A4 Protein (rp145636) with the EC50 of 8.848 ng/mL.
表达系统
HEK293
种属
人(Human)
氨基酸
1-547 aa
序列
MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTHHHHHH
蛋白标签
C-His
无载体
Yes
无动物源
Yes
来源
重组表达
预测分子量
59.2 kDa
SDS-PAGE
66.2 kDa, under reducing conditions; 61.1 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
biological_products
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片

Recombinant Human EphA4 Protein (rp183616) - Binding Activity
Immobilized Recombinant Human EphA4 Protein (rp183616) at 2.0 μg/mL can bind Human Recombinant Ephrin-A4 Protein (rp145636) with the EC₅₀ of 8.85 ng/mL.

Recombinant Human EphA4 Protein (rp183616) - SDS-PAGE
3 μg/lane of Recombinant Human EphA4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 66.2 kDa under reducing conditions and 61.1 kDa under non-reducing conditions

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

质检证书(CoA,COO,BSE/TSE 和分析图谱)
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ24F0809265 分析证书 rp183616
ZJ24F0809266 分析证书 rp183616
ZJ24F0809267 分析证书 rp183616
技术文档和文章
溶液计算器