Neprilysin/CD10, also known as NEP and neutral endopeptidase 24.11, is a zinc metallopeptidase expressed at the cell surface of a variety of cells. The enzyme functions both as an endopeptidase with a thermolysin-like specificity and as a dipeptidylcarboxypeptidase. NEP has been shown to be involved in the degradation of enkephalins in the mammalian brain and the inactivation of circulating atrial natriuretic peptide. NEP has also been identified as the common acute lymphocytic leukemia antigen (CALLA), and is expressed on the surface of lymphocytes in some disease states. These and other observations have resulted in considerable interest in NEP as a target for analgesics and antihypertensive drugs. NEP is also a major degrading enzyme of amyloid beta peptide (Aβ) in the brain, indicating that down-regulation of NEP activity, which could be caused by aging, can contribute to the development of Alzheimer’s disease by promoting Aβ accumulation.
Post-translational: Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.
生物活性
Immobilized Recombinant Human Neprilysin Protein (rp183630) at 1.0 μg/mL can bind CD10 Mouse mAb (Ab093617) with the EC50 of 109.3 ng/mL.
81.3-100.1 kDa, under reducing conditions; 81.3-100.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM MES, 100mM NaCl, 5% Glycerol, pH 6.5
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Neprilysin Protein (rp183630) - ELISA Immobilized Recombinant Human Neprilysin Protein (rp183630) at 1.0 μg/mL can bind CD10 Mouse mAb (Ab093617) with the EC₅₀ of 109.3 ng/mL.
Recombinant Human Neprilysin Protein (rp183630) - SDS-PAGE 3 μg/lane of Recombinant Human Neprilysin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 81.3-100.1 kDa.