Recombinant Human Nectin-4 Protein

无动物源

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

功能和特点

表达系统: HEK293
Accession #: Q96NY8-1
蛋白标签: C-His
生物活性: Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC50 of 17.59 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp183631-10μg (试用装) 申请此免费试用装（?）作为尊贵的客户，您每年可免费申请一次试用装产品，尽享探索与享受！	10μg	现货
rp183631-50μg	50μg	现货
rp183631-100μg	100μg	现货
rp183631-1mg	1mg	期货

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点击查看蛋白信息: NECTIN4

Accession#: Q96NY8-1 (1) Gene ID:81607 (3) Nectin-4 (2) NECTIN4 (3)

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基本描述

产品名称	Recombinant Human Nectin-4 Protein
别名	重组人Nectin-4蛋白 \| 重组人类 Nectin-4 蛋白
英文别名	Ig superfamily receptor LNIR \| EDSS1 \| LNIR \| LNIRnectin 4 \| Nectin4 \| Nectin-4 \| poliovirus receptor-related 4 \| poliovirus receptor-related protein 4 \| PRR4 \| PRR4DKFZp686K05193 \| PVRL4
规格或纯度	ActiBioPure™, 生物活性, 无动物源, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function:： Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The Latin word necto means “to connect”, indicating the role of nectins in Ca2+‑independent cell-cell adhesion. Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4. Human Nectin-4 mRNA is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain. Human Nectin-4 cDNA encodes 510 amino acids (aa), including a 31 aa signal sequence, a 318 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type. One Nectin‑4 isoform lacks aa 412-436 in the cytoplasmic domain. In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is upregulated and a soluble 43 kDa form is found in the plasma . This form is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins. These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development.
生物活性	Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC50 of 17.59 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-349 aa
序列	MPLSLGAEMWGPEAWLLLLLLLASFTGRCPAGELETSDVVTVVLGQDAKLPCFYRGDSGEQVGQVAWARVDAGEGAQELALLHSKYGLHVSPAYEGRVEQPPPPRNPLDGSVLLRNAVQADEGEYECRVSTFPAGSFQARLRLRVLVPPLPSLNPGPALEEGQGLTLAASCTAEGSPAPSVTWDTEVKGTTSSRSFKHSRSAAVTSEFHLVPSRSMNGQPLTCVVSHPGLLQDQRITHILHVSFLAEASVRGLEDQNLWHIGREGAMLKCLSEGQPPPSYNWTRLDGPLPSGVRVDGDTLGFPPLTTEHSGIYVCHVSNEFSSRDSQVTVDVLDPQEDSGKQVDLVSASHHHHHH
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q96NY8-1
来源	重组表达
预测分子量	34.9 kDa
SDS-PAGE	39.8 kDa, under reducing conditions; 37.4 kDa, under non-reducing conditions

图片

ELISA: Recombinant Human Nectin-4 Protein (rp183631)

Recombinant Human Nectin-4 Protein (rp183631) - ELISA
Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC₅₀ of 17.59 ng/mL.

SDS-PAGE: Recombinant Human Nectin-4 Protein (rp183631)

Recombinant Human Nectin-4 Protein (rp183631) - SDS-PAGE
3 μg/lane of Recombinant Human Nectin-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.8 kDa under reducing conditions and 37.4 kDa under non-reducing conditions.

产品规格参数

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

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批号(Lot Number)	证书类型	日期	货号
ZJ24F0809273	分析证书	24-08-08	rp183631
ZJ24F0809272	分析证书	24-08-08	rp183631
ZJ24F0809271	分析证书	24-08-08	rp183631

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Recombinant Human Nectin-4 Protein

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

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