Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC50 of 17.59 ng/mL.
Protein Function:： Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. The Latin word necto means “to connect”, indicating the role of nectins in Ca2+‑independent cell-cell adhesion. Nectin-4 forms homodimers in cis, followed by interactions in trans with Nectin-1 or -4. Human Nectin-4 mRNA is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain. Human Nectin-4 cDNA encodes 510 amino acids (aa), including a 31 aa signal sequence, a 318 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 140 aa cytoplasmic region. Nectin ECDs contain three Ig-like domains: an N‑terminal V-type that mediates ligand binding, and two C2-type. One Nectin‑4 isoform lacks aa 412-436 in the cytoplasmic domain. In many human ductal breast or non-small cell lung carcinomas, Nectin‑4 is upregulated and a soluble 43 kDa form is found in the plasma . This form is generated from the membrane protein via the action of TACE/ADAM-17. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90% amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions, trans interactions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins. These interactions organize the actin cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. In humans, mutation of Nectin-4 has been correlated with ectodermal dysplasia-syndactyly syndrome, indicating a role for Nectin-4 in human development.
Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC50 of 17.59 ng/mL.
39.8 kDa, under reducing conditions; 37.4 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human Nectin-4 Protein (rp183631) - ELISA Immobilized Recombinant Human Nectin-4 Protein (rp183631) at 1.0 μg/mL can bind Enfortumab vedotin-ejfv (anti-Nectin-4) (Ab175528) with the EC₅₀ of 17.59 ng/mL.
Recombinant Human Nectin-4 Protein (rp183631) - SDS-PAGE 3 μg/lane of Recombinant Human Nectin-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.8 kDa under reducing conditions and 37.4 kDa under non-reducing conditions.
ZJ24F0809271
危险品化学品经营许可证（带存储）