ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a member of the ROR family of receptor tyrosine kinases and is important for skeletal development, including bone and cartilage formation, as well as for the development of the central nervous system. Mature human ROR2 contains a 369 amino acid (aa) extracellular domain (ECD) and a 518 aa cytoplasmic tail containing a tyrosine kinase domain. The ROR2 ECD features an immunoglobulin domain, a cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, and a kringle domain. The ECD of human ROR2 shares 93% and 94% aa sequence identity with the ECD of mouse and rat ROR2, respectively. ROR2 binds the Wnt family ligand, Wnt-5a, to activate non-canonical Wnt signaling pathways. ROR2 is broadly expressed during embryonic development and can be found in cells of all three germ layers as well as in most organ tissues. Activation of ROR2 signaling promotes cellular proliferation, differentiation, cell-polarization, and migration. ROR2 is important for osteogenic and chondrogenic differentiation of mesenchymal stem cells. Mutations in ROR2 are associated with the skeletal disorders, brachydactyly type B and Robinow syndrome. ROR2 is also involved in neurite outgrowth and synapse development in the brain. A variety of tumors have been found to over-express ROR2. Depending upon the cellular context, ROR2 can act as a tumor suppressor or promoter. Expression of ROR2 within carcinomas is correlated with a poor prognosis of survival and increased rates of recurrence.
生物活性
Immobilized Recombinant Human ROR2 Protein (rp183636) at 1.0 μg/mL can bind Ozuriftamab vedotin (anti-ROR2) (Ab183322) with the EC50 of 120.8 ng/mL.
49.6-57.0 kDa, under reducing conditions; 44.8-50.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human ROR2 Protein (rp183636) - ELISA Immobilized Recombinant Human ROR2 Protein (rp183636) at 1.0 μg/mL can bind Ozuriftamab vedotin (anti-ROR2) (Ab183322) with the EC50 of 120.8 ng/mL.
Recombinant Human ROR2 Protein (rp183636) - SDS-PAGE 3 μg/lane of Recombinant Human ROR2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 49.6-57.0 kDa under reducing conditions and 44.8-50.7 kDa under non-reducing conditions.