Immobilized Recombinant Human SMAC/Diablo Protein (rp183671) at 1.0 μg/mL can bind Recombinant Smac/Diablo Antibody (Ab128141) with the EC50 of 132.8 ng/mL.
0610041G12Rik | Diablo | diablo, IAP-binding mitochondrial protein | Direct IAP-binding protein with low pI | mitochondrial Smac protein | Second mitochondria-derived activator of caspase | SMAC | SMACmitochondrial
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE)
生化机理
Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer.
Post-translational: Ubiquitinated by BIRC7/livin. Ubiquitinated by BIRC6. The precursor form is proteolytically cleaved by mitochondrial processing peptidase MPP to remove the transit peptide and produce an intermediate form. This is then processed by PARL to produce the mature cleaved form which is released from mitochondria into the cytosol in apoptotic cells.
生物活性
Immobilized Recombinant Human SMAC/Diablo Protein (rp183671) at 1.0 μg/mL can bind Recombinant Smac/Diablo Antibody (Ab128141) with the EC50 of 132.8 ng/mL.
20.6 kDa, under reducing conditions; 20.6 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20 mM HEPES, 150 mM NaCl, pH 7.5.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human SMAC/Diablo Protein (rp183671) - ELISA Immobilized Recombinant Human SMAC/Diablo Protein (rp183671) at 1.0 μg/mL can bind Recombinant SMAC/Diablo Antibody (Ab128141) with the EC50 of 132.8 ng/mL.
Recombinant Human SMAC/Diablo Protein (rp183671) - SDS-PAGE 3 μg/lane of Recombinant Human SMAC/Diablo Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 20.6 kDa.
ZJ24F1215379
危险品化学品经营许可证（带存储）