级别和纯度: 无载体?无载体（Carrier Free）—— 不含添加的载体蛋白/稳定剂。当载体（如 BSA）会干扰偶联或敏感检测时使用。生物活性?生物活性级（Bioactive）—— 经验证在功能检测中保持生物活性。当分子须具有功能活性而不仅是纯度时使用。ActiBioPure™?ActiBioPure™ —— 阿拉丁面向生物活性和重组产品的优质系列。当同时需要高纯度和保持生物活性时使用。高性能?高性能级 —— 纯度和性能特性经过优化。适用于普通等级无法满足的灵敏分析。His标签?组氨酸(His) 标签级 —— 带有 His 标签的重组蛋白，供亲和纯化/检测。用于纯化、固定或检测带标签蛋白。≥90%(SDS-PAGE)expressed in E. coli; 见COA
Accession #
P12034
蛋白标签
C-His
表达系统
E. coli
内毒素水平
<1.0 EU/μg
生物活性
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3.9 ng/mL in the presence of 1 µg/mL of heparin.
无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥90%(SDS-PAGE), expressed in E. coli; 见COA
生化机理
The FGF family is comprised of at least seven polypeptides that are potent regulators of cell proliferation, differentiation and function. All FGFs have two conserved cysteine residues and share 30 - 50% sequence homology at the amino acid level. FGF-5 was originally identified as a transforming gene by the NIH-3T3 focus formation assay using DNA derived from human tumors. FGF-5 cDNA encodes a 267 amino acid residue protein with a putative 22 amino acid residue signal peptide. The murine homologue of FGF-5 was cloned and found to be 84% homologous to the human protein at the amino acid sequence level. Human and murine FGF-5 exhibit cross species activity.
In vitro, rhFGF-5 is a mitogen for Balb/3T3 fibroblasts and bovine heart endothelial cells. FGF-5 was also reported to be a major muscle-derived survival factor for cultured spinal motoneurons. In vivo, FGF-5 is suggested to play important roles in both embryology and neurobiology. Developmentally, FGF-5 mRNA is initially found in the embryoblast followed by the lateral somatic mesoderm, where it may play a role in angiogenesis, plus the myotomes cranial to the tail region, where it may delay terminal myoblast differentiation during cell migration. FGF-5 continues to impact muscle post-natally where it is believed to function as a target-derived neurotrophic factor of skeletal muscle. In the nervous system, FGF-5 has been most often identified in neurons associated with the limbic system, notably in neurons of the olfactory bulb and pyramidal cells of the hippocampus. Hippocampal FGF-5 is suggested to serve as a neurotrophic and differentiative factor for cholinergic and serotonergic neurons projecting to this region.
生物活性
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3.9 ng/mL in the presence of 1 µg/mL of heparin.
Lyophilized from PBS, 5% Trehalose, 5% Mannitol, pH 7.4
浓度
expressed in E. coli; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human FGF-5 Protein (rp183679) - SDS-PAGE
3 μg/lane of Recombinant Human FGF-5 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing the bands at 30.0 & 57.2 kDa under reducing conditions.
Unique among FGFs, ligands of the FGF9 subfamily (FGF9, FGF18, FGF20) undergo reversible homodimerization mediated by extensive hydrophobic contacts, hydrogen bonds and van der Waals interactions, independent of disulfide bonds. Closely related members in the FGF4/5/6 clade share conserved surface hydrophobic patches and exhibit analogous concentration-dependent self-association behavior (PMID: 36905676).
Recombinant Human FGF-5 Protein (rp183679) - Protein Bioactivity
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3.9 ng/mL in the presence of 1 µg/mL of heparin.
危险品化学品经营许可证（带存储）