Protein phosphatase 1A (PPM1A / PP2CA) is an enzyme belonging to the PP2C family of Ser / Thr protein phosphatases. Members of PP2C family are negative regulators of cell stress response pathways and the MAP kinases and MAP kinase kinases. It has also been demonstrated to inhibit the activation of p38 and JNK kinase cascades. PPM1A dephosphorylates and promotes nuclear export of TGFβ-activated Smad2/3. Ectopic expression of PPM1A abolishes TGFβ-induced antiproliferative and transcriptional responses, whereas depletion of PPM1A enhances TGFβ signaling in mammalian cells. It has been demonstrated that PPM1A / PP2CA, through dephosphorylation of Smad2/3, plays a critical role in terminating TGFβ signaling. Overexpression of PPM1A is reported to activate the expression of the tumor suppressor gene TP53 / p53, which leads to cell apoptosis.
Post-translational: N-myristoylation is essential for the recognition of its substrates for dephosphorylation.
生物活性
Immobilized Recombinant Human PPM1A Protein (rp183695) at 1.0 μg/mL can bind PPM1A Antibody (Ab122785) with the EC50 of 21.2 ng/mL.
39.1 kDa, under reducing conditions; 39.1 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 10% Glycerol, pH 7.5
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human PPM1A Protein (rp183695) - ELISA Immobilized Recombinant Human PPM1A Protein (rp183695) at 1.0 μg/mL can bind PPM1A Antibody (Ab122785) with the EC₅₀ of 21.2 ng/mL.
Recombinant Human PPM1A Protein (rp183695) - SDS-PAGE 2 μg/lane of Recombinant Human PPM1A Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 39.1 kDa.