Immobilized Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) at 1.0 μg/mL can bind UBE2M/UBC12 Mouse mAb (Ab133254) with the EC50 of 25.4 ng/mL.
EC 6.3.2 | EC 6.3.2.- | hUbc12 | NEDD8 carrier protein | NEDD8 protein ligase | UBC12 | UBC12NEDD8-conjugating enzyme Ubc12 | UbcH12 | UBC-RS2 | UBE2M | ubiquitin carrier protein M | Ubiquitin-conjugating enzyme E2 M | ubiquitin-conjugating enzyme E2M (ho
规格或纯度
无载体, ActiBioPure™, 生物活性, ≥95%(SDS-PAGE), 见COA
生化机理
Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins. Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme. The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression. UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family.
Post-translational: The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
生物活性
Immobilized Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) at 1.0 μg/mL can bind UBE2M/UBC12 Mouse mAb (Ab133254) with the EC50 of 25.4 ng/mL.
19.2 kDa, under reducing conditions; 19.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
PBS, 10% Glycerol, 1mM DTT, pH 8.0
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃ 存储6个月；收货后建议分装，避免反复冻融。
图片
Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) - SDS-PAGE 3 μg/lane of Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 19.2 kDa.
Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) - ELISA Immobilized Recombinant Human/Mouse/Rat UBE2M/Ubc12 Protein (rp183713) at 1.0 μg/mL can bind UBE2M/UBC12 Mouse mAb (Ab133254) with the EC₅₀ of 25.4 ng/mL.
危险品化学品经营许可证（带存储）