Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. The ED50 for this effect is 2.679 μg/mL.
Cadherins are calcium-dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass transmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. The ED50 for this effect is 2.679 μg/mL.
83.5 & 102.3 kDa, under reducing conditions; 76.8 & 98.0 kDa, under non-reducing conditions
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human N-Cadherin Protein (rp183731) - Protein Bioactivity Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. The ED₅₀ for this effect is 2.679 μg/mL.
Recombinant Human N-Cadherin Protein (rp183731) - SDS-PAGE 3 μg/lane of Recombinant Human N-Cadherin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 83.5 & 102.3 kDa under reducing conditions and 76.8 & 98.0 kDa under non-reducing conditions.