无动物源,无载体,Fc标签,≥95%(SDS-PAGE),expressed in CHO; 见COA
生化机理
Frizzled-5 is an approximately 62 kDa 7-TM glycoprotein that is one of at least ten Frizzled family Wnt receptors. Wnt engagement of Frizzled, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co‑receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Frizzleds can also signal through non-canonical pathways independently of LRPs. Mature human Frizzled-5 consists of a 212 amino acid (aa) extracellular domain (ECD), a 283 aa 7-TM region, and a 64 aa cytoplasmic domain with a PDZ binding motif. The ECD includes a cysteine-rich region (CRD) that binds Wnts and is highly conserved among Frizzled proteins. Within the N-terminal ECD, human Frizzled-5 shares 95% aa sequence identity with mouse and rat Frizzled-5. Frizzled-5 is expressed in embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney), in the adult retina, colon, and pancreatic islets, some cancer cell lines, human embryonic stem cells, and in some monocyte and lymphocyte populations. Frizzled-5 functions as a receptor for Wnt-5a, Wnt-9b, Wnt-10b, Wnt-2b, and Wnt-7a. It plays a role in the maintenance of yolk sac and placental vasculature (ishikawa) and in the regression of vitreous vasculature during eye development. It mediates the synaptogenic effect of Wnt-7a, contributes to the development of neuronal polarity, and is required for neuronal survival in the thalamus. In macrophages and monocytes, Frizzled-5 signaling induces the production of inflammatory cytokines.
Post-translational: Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome.
48.4-59.8 kDa, under reducing conditions; 104.8-126.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in CHO; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human Frizzled-5 Protein (rp184036) - SDS-PAGE
3 μg/lane of Recombinant Human Frizzled-5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 48.4-59.8 kDa under reducing conditions and 104.8-126.2 kDa under non-reducing conditions.
Under non-reducing SDS-PAGE, Frizzled-5 forms stable homodimers, which are stabilized by intramolecular disulfide bonds in the CRD and non-covalent interactions between CRD and transmembrane domains (PMID: 28377511; PMID: 32762848).
ZJ26F0535506
危险品化学品经营许可证（带存储）