Semaphorin 7A (Sema7A, designated CD108, previously Sema K1 or Sema L), is an ~80 kDa membrane-anchored glycoprotein that is a member of the Semaphorin family of axon guidance molecules. On human erythrocytes, it is the John Milton Hagen (JMH) blood group antigen. Sema7A is the only known Class 7 or glycophosphatidylinositol (GPI)-linked semaphorin; its expression is concentrated in the brain, spleen, and thymus. Human Sema7A cDNA encodes a 44 amino acid (aa) signal sequence, a 604 aa extracellular domain (ECD) including Sema and C2‑type Ig-like domains, and an 18 aa propeptide/GPI membrane anchor signal sequence. Mature human Sema7A shares 89%, 89%, 88%, 86%, and 90% aa identity with mouse, rat, canine, bovine, and equine Sema7A, respectively. The Sema7A sema domain contains an RGD integrin interaction motif. Although it binds plexin-C1 in vitro and may be coexpressed with it, many of its activities depend on interaction with beta 1 integrins such as alpha 1 beta 1. Sema7A signaling through the two receptors may cause opposing effects. Sema7A is an immune semaphorin, with expression and activity on CD4+CD8+ thymocytes, activated T cells, macrophages, and microglia. T cell Sema7A interacts with monocytic cells, stimulating their chemotaxis, production of pro-inflammatory cytokines, and dendritic differentiation. However, on the T cells themselves, Sema7A downregulates TCR signaling by promoting TCR internalization, modulating T cell responses. In lung macrophages, Sema7A is induced by TGF-beta and participates in TGF-beta-induced lung fibrosis. Sema7A is also expressed on pre-osteoblasts and osteoclasts, where it promotes migration and fusion, respectively; on keratinocytes, where it promotes melanocyte spreading and dendricity; and on some neurons, for example, promoting axon outgrowth in the developing olfactory tract.
生物活性
Immobilized Recombinant Human Semaphorin 7A Protein (rp184082) at 2.0 μg/mL can bind Semaphorin 7a Antibody (Ab127023) with the EC50 of 5.2 ng/mL.
73.0 kDa, under reducing conditions; 72.1 & 128.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Semaphorin 7A Protein (rp184082) - ELISA Immobilized Recombinant Human Semaphorin 7A Protein (rp184082) at 2.0 μg/mL can bind Semaphorin 7a Antibody (Ab127023) with the EC50 of 5.2 ng/mL.
Recombinant Human Semaphorin 7A Protein (rp184082) - SDS-PAGE 3 μg/lane of Recombinant Human Semaphorin 7A Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 73.0 kDa under reducing conditions and 72.1 & 128.0 kDa under non-reducing conditions. Sema7A is a homodimeric glycoprotein (Uniprot ID: O75326).
ZJ25F0318991
危险品化学品经营许可证（带存储）