CPI-B | CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B | CSTB | cystatin B (stefin B) | Cystatin B | EPM1 | Liver thiol proteinase inhibitor | PME | Stefin B | stefin-B
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE)
生化机理
Cystatin-B, also known as CPI-B, Liver thiol proteinase inhibitor, Stefin-B, CSTB and CST6, is a cytoplasm and nucleus protein which belongs to thecystatin family. Cystatin-B / CSTB is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. Cystatin-B / CSTB is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Cystatin-B / CSTB is also thought to play a role in protecting against the proteases leaking from lysosomes. Defects in Cystatin-B / CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) which is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The cystatins are a family of cysteine protease inhibitors with homology to chicken cystatin. Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. Cystatins typically comprise about 115 amino acids, are largely acidic, contain four conserved cysteine residues known to form two disulfide bonds. Cystatins may be glycosylated and / or phosphorylated, with similarity to fetuins, kininogens, stefins, histidine-rich glycoproteins and cystatin-related proteins. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired inhibitory activity. Cystatins mainly inhibit peptidases belonging to peptidase families C1 (papain family) and C13 (legumain family).
生物活性
Immobilized Recombinant Human Cystatin-B Protein (rp184103) at 1.0 μg/mL can bind Cystatin-B Antibody (Ab098665) with the EC50 of 4.3 ng/mL.
12.8 & 28.6 kDa, under reducing conditions; 13.0 & 27.7 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 50mM Tris, 50mM NaCl, pH 8.0.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Cystatin-B Protein (rp184103) - ELISA Immobilized Recombinant Human Cystatin-B Protein (rp184103) at 1.0 μg/mL can bind Cystatin-B Antibody (Ab098665) with the EC50 of 4.3 ng/mL.
Recombinant Human Cystatin-B Protein (rp184103) - SDS-PAGE 3 μg/lane of Recombinant Human Cystatin-B Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.8 & 28.6 kDa under reducing conditions and 13.0 & 27.7 kDa under non-reducing conditions.