Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage where the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner. Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogates both phosphorylation of p53 on Serine 20 and p53 stabilization. Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
Post-translational: Phosphorylated by PLK4.
SDS-PAGE: 63.5 & 156.8 kDa, under reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from 20mM Tris, 500mM NaCl, 2M Urea, pH 8.0.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Chk2 Protein (rp184105) - SDS-PAGE 3 μg/lane of Recombinant Human Chk2 Protein was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing a band at 63.5 & 156.8 kDa.
ZJ26F0738036
危险品化学品经营许可证（带存储）