The ZAP70 (ζ-associated protein of 70 kDa) is expressed in the aggressive form of B-cell chronic lymphocytic leukemia (CLL). ZAP70 plays a critical role for ZAP70 in chemokine-mediated, inside-out signaling to integrins in trisomy. The phosphorylation of ZAP70 activated the downstream NF-κB signaling pathway, which is involved in both T cell growth and function. On the other hand, the expression of Zap70 in B-cell malignancies correlates with the severity of the disease. Because of its role in immune-related disorders, Zap70 has become a therapeutic target for the treatment of human diseases.
Post-translational: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive function on T-lymphocyte activation whereas Tyr-292 has a negative regulatory role. Within the C-terminal kinase domain, Tyr-492 and Tyr-493 are phosphorylated after TCR induction, Tyr-492 playing a negative regulatory role and Tyr-493 a positive. Tyr-493 is dephosphorylated by PTN22. Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human ZAP70 Protein (rp184111) - SDS-PAGE 3 μg/lane of Recombinant Human ZAP70 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 27.6 kDa under reducing conditions.
危险品化学品经营许可证（带存储）