CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
规格
产品名称
Recombinant Human CDK1 Protein
别名
重组人CDK1蛋白 | 重组人类 CDK1 蛋白
英文别名
Cdc2 | Cdc 2 | CDC28A | CDK 1 | CDK1 | CDK1_HUMAN | CDKN1 | CELL CYCLE CONTROLLER CDC2 | Cell division control protein 2 | Cell division control protein 2 homolog | Cell division cycle 2 G1 to S and G2 to M | Cell division protein kinase 1 | Cell Divsion
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥90%(SDS-PAGE)
生物活性
Immobilized Recombinant Human CDK1 Protein (rp184120) at 1.0 μg/mL can bind Recombinant CDK1 Antibody (Ab095719) with the EC50 of 3.0 ng/mL.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
图片
Recombinant Human CDK1 Protein (rp184120) - ELISA Immobilized Recombinant Human CDK1 Protein (rp184120) at 1.0 μg/mL can bind Recombinant CDK1 Antibody (Ab095719) with the EC₅₀ of 3.0 ng/mL.
Recombinant Human CDK1 Protein (rp184120) - SDS-PAGE 3 μg/lane of Recombinant Human CDK1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 31.6 kDa.
ZJ24F0809870
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