HSMT 3 | HSMT3 | MGC117191 | Sentrin 2 | Sentrin-2 | Sentrin2 | Small ubiquitin like modifier 2 | Small ubiquitin related modifier 2 | Small ubiquitin-related modifier 2 | SMT 3B | SMT3 homolog 2 | SMT3 suppressor of mif two 3 homolog 2 | SMT3 suppressor
规格或纯度
无载体,His标签,≥95%(SDS-PAGE)
生化机理
Ubiquitin-like protein that can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451.
This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Polymeric SUMO2 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins.
Plays a role in the regulation of sumoylation status of SETX.
Post-translational: Polymeric chains can be formed through Lys-11 cross-linking. Polymeric SUMO2 chains undergo 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination by RNF4.
Cleavage of precursor form by SENP1 or SENP2 is necessary for function.
Monoubiquitinated N-terminally by UBE2W, which primes it for RNF4-dependent polyubiquitination by the UBE2V1-UBE2N heterodimer.
15.4 kDa, under reducing conditions; 15.6 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 10% Trehalose, pH 7.4.
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human SUMO2 Protein (rp184857) - SDS-PAGE 3 μg/lane of Recombinant Human SUMO2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.4 kDa under reducing conditions and 15.6 kDa under non-reducing conditions.
ZJ26F0838568
危险品化学品经营许可证（带存储）