Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor. The specific activity is >225.0 pmol/min/μg, as measured under the described conditions.
Clearance of heme in mammals is a two-step process starting with the conversion of heme to biliverdin by heme oxygenase, followed by the reduction of biliverdin to bilirubin by biliverdin reductase. Biliverdin IX b reductase (BLVRB) converts the beta isomer of biliverdin IX to bilirubin IX b, which constitutes 87% of total bilirubin in fetal bile. Therefore, BLVRB is especially important for fetal heme metabolism and clearance. BLVRB is a cytoplasmic enzyme expressed at high levels in erythrocytes and liver, but it is present in other tissues. The enzyme is identical to flavin reductase, which is an oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD, FMN, and met-hemoglobin. BLVRB is structurally distinct from BLVRA. In contrast to BLVRA, which prefers the biliverdin alpha isomer but could also use the beta isomer as a substrate, BLVRB is specific for the beta isomer.
生物活性
Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor. The specific activity is >225.0 pmol/min/μg, as measured under the described conditions.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human Biliverdin Reductase B/BLVRB Protein (rp184878) - SDS-PAGE 3 μg/lane of Recombinant Human Biliverdin Reductase B/BLVRB Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 24.8 kDa.
ecombinant Human Biliverdin Reductase B/BLVRB Protein - Enzyme Activity Measured by the reduction of riboflavin 5'-monophosphate (FMN) using NADPH as the cofactor. The specific activity is >225 pmol/min/μg, as measured under the described conditions. Materials: Assay Buffer: 100 mM Sodium Acetate, pH 5.0 Recombinant Human Biliverdin Reductase B/BLVRB Protein (rp184878) Riboflavin 5’-monophosphate sodium salt dihydrate (FMN) (R421185), 10 mM in deionized water beta -Nicotinamide adenine dinucleotide phosphate reduced, tetrasodium salt ( beta -NADPH) (N276326), 10 mM in deionized water Procedures： 1. Dilute rhBLVRB to 4.4 ng/μL in Assay Buffer. 2. Prepare a Reaction Mixture by combining FMN and beta -NADPH in Assay Buffer to a concentration of 3.2 mM for each. 3. Load 50 μL of 4.4 ng/μL rhBLVRB into the microplate, and start the reaction by adding 50 μL of Reaction Mixture. Include a Substrate Blank containing 50 μL of Assay Buffer and 50 μL of Reaction Mixture. 4. Read at an absorbance of 340 nm in kinetic mode for 5 minutes (Note: readings will be negative). 5. Calculate specific activity:
*Adjusted for Substrate Blank
**Using the extinction coefficient 6270 M-1cm-1
***Using the path correction 0.32 cm Note: the output of many spectrophotometers is in mOD Per Well: rhBLVRB: 0.22 μg, FMN: 1.6 mM, beta -NADPH: 1.6 mM
ZJ25F0521211
危险品化学品经营许可证（带存储）