Catechol-O-methyltransferase (COMT) catalyzes the transfer of the methyl group from S-adenosyl-L-methionine to either the meta- or the para-hydroxyl group of the catechol ring from a variety of endogenous and exogenous catechol substrates including the neurotransmitters dopamine, epinephrine, and norepinephrine. Thus, it plays important roles in one of the major degradative pathways of the catecholamine transmitters, and in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson’s disease. In tissues, COMT is produced as two distinct forms, a soluble protein (residues 52-271) and a membrane-bound protein that possesses the N-terminal transmembrane domain. A common single-nucleotide polymorphism results in substitution of methionine for valine at position 158 of membrane-bound COMT and position 108 at soluble COMT. This amino acid change results in a more unstable and three- to four-fold less active 108/158Met form. Since COMT activity is the major regulator of dopamine function in the prefrontal brain region, the Val108/158Met variation is considered to be associated with increased risk of a wide spectrum of mental disorders.
Post-translational: The N-terminus is blocked.
生物活性
Immobilized Recombinant Human COMT Protein (rp184883) at 1.0 μg/mL can bind COMT Antibody (Ab097283) with the EC50 of 5.6 ng/mL.
24.4 kDa, under reducing conditions; 24.4 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
液体
储存缓冲液
20mM Tris, 1mM MgCl2, 10% Glycerol, pH 8.0
浓度
见COA
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human COMT Protein (rp184883) - ELISA Immobilized Recombinant Human COMT Protein (rp184883) at 1.0 μg/mL can bind COMT Antibody (Ab097283) with the EC50 of 5.6 ng/mL.
Recombinant Human COMT Protein (rp184883) - SDS-PAGE 3 μg/lane of Recombinant Human COMT Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 24.4 kDa.