Ataxia telangiectasia and Rad3 related | Ataxia telangiectasia and Rad3-related protein | ATR | ATR_HUMAN | FCTCS | FRAP Related Protein 1 | FRAP-related protein 1 | FRP1 | MEC1 | MEC1 mitosis entry checkpoint 1 homolog | Protein kinase ATR | RAC3 | Rad3
规格或纯度
无载体,His标签,≥90%(SDS-PAGE),见COA
生化机理
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.
Post-translational: Phosphorylated (PubMed:21144835). Autophosphorylation at Thr-1989 in response to DNA damage promotes interaction with TOPBP1 and activation of ATR (PubMed:21705319, PubMed:21777809).
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human ATR Protein (rp184888) - SDS-PAGE 3 μg/lane of Recombinant Human ATR Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 30.9 kDa under reducing conditions and 30.0 & 60.3 kDa under non-reducing conditions. ATR Protein can form homodimers through its C-terminal domain, without the involvement of ATRIP (PMID: 35721505).
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