Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 | ENPP3 | Phosphodiesterase I beta | PD-Ibeta | CD203c | NPP3 | E-NPP3 | NPPase | PDNP3 | B10
规格或纯度
无动物源,无载体,His标签,≥95%(SDS-PAGE),expressed in HEK293; 见COA
生化机理
The human NPP family contains seven members which can be classified into two groups according to their substrate preferences. The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4. NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells. Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface. This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry. Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions. Following activation, these cells release ATP, which further stimulates them in an autocrine manner. NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).
Post-translational: N-glycosylated. N-glycosylation is necessary for normal transport to the cell membrane, but is not the apical targeting signal.
111.7-139.3 kDa; under reducing conditions; 107.1-137.4 kDa; under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
expressed in HEK293; 见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human ENPP3 Protein (rp184894) - SDS-PAGE
2 μg/lane of Recombinant Human ENPP3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 111.7-139.3 kDa under reducing conditions and 107.1-137.4 kDa under non-reducing conditions.
The two bands of ENPP3/B10 may correspond to its open and closed conformational states with distinct structural compactness (PMID: 29717535).
危险品化学品经营许可证（带存储）